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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Diabetes, Physiology and Molecular Medicine Research Group > Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/6310
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Title: Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture
Authors: Seyffarth, Gunther
Nelson, Paul N.
Dunmore, Simon J.
Rodrigo, Nalinda
Murphy, Damian J.
Carson, Ray J.
Citation: Reproductive Biology and Endocrinology, 2: 29
Publisher: BioMed Central
Issue Date: 2004
URI: http://hdl.handle.net/2436/6310
DOI: 10.1186/1477-7827-2-29
Additional Links: http://www.rbej.com/content/2/1/29
Abstract: Platelet-activating factor and nitric oxide may be involved in the initiation of human labour as inflammatory mediators. The aim of this study was to test whether platelet-activating factor and lipopolysaccharide were able to induce nitric oxide synthase expression and stimulate the production of nitric oxide in human fetal membrane explants in culture. Fetal membranes were collected from Caesarean sections at term. RNA was extracted from membranes and subjected to a qualitative RT-PCR to assess the baseline expression of iNOS. Discs of fetal membranes were cultured for 24 hours in the presence of platelet-activating factor at a dose range of 0.1 nanomolar – 1 micomolar or 1 microgram/ml lipopolysaccharide. Nitric oxide production was measured via nitrite ions in the culture medium and mRNA for iNOS was detected by RT-PCR. Culturing the membrane discs in medium containing serum induced nitric oxide synthase expression and platelet-activating factor significantly stimulated the production of nitric oxide under these conditions. When cultured without serum inducible nitric oxide synthase expression was induced by lipopolysaccharide, but not by platelet-activating factor. Platelet-activating factor may have a role in the initiation of labour, at term or preterm, via the increased local production of nitric oxide as an inflammatory mediator. In this model of intrauterine infection, lipopolysaccharide was found to induce iNOS expression by fetal membranes, and this mechanism could be involved in preterm labour.
Type: Article
Language: en
Keywords: Lipopolysaccharide
Human fetal membranes
Nitric oxide
ISSN: 14777827
Appears in Collections: Diabetes, Physiology and Molecular Medicine Research Group

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