Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture

2.50
Hdl Handle:
http://hdl.handle.net/2436/6310
Title:
Lipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in culture
Authors:
Seyffarth, Gunther; Nelson, Paul N.; Dunmore, Simon J.; Rodrigo, Nalinda; Murphy, Damian J.; Carson, Ray J.
Abstract:
Platelet-activating factor and nitric oxide may be involved in the initiation of human labour as inflammatory mediators. The aim of this study was to test whether platelet-activating factor and lipopolysaccharide were able to induce nitric oxide synthase expression and stimulate the production of nitric oxide in human fetal membrane explants in culture. Fetal membranes were collected from Caesarean sections at term. RNA was extracted from membranes and subjected to a qualitative RT-PCR to assess the baseline expression of iNOS. Discs of fetal membranes were cultured for 24 hours in the presence of platelet-activating factor at a dose range of 0.1 nanomolar – 1 micomolar or 1 microgram/ml lipopolysaccharide. Nitric oxide production was measured via nitrite ions in the culture medium and mRNA for iNOS was detected by RT-PCR. Culturing the membrane discs in medium containing serum induced nitric oxide synthase expression and platelet-activating factor significantly stimulated the production of nitric oxide under these conditions. When cultured without serum inducible nitric oxide synthase expression was induced by lipopolysaccharide, but not by platelet-activating factor. Platelet-activating factor may have a role in the initiation of labour, at term or preterm, via the increased local production of nitric oxide as an inflammatory mediator. In this model of intrauterine infection, lipopolysaccharide was found to induce iNOS expression by fetal membranes, and this mechanism could be involved in preterm labour.
Citation:
Reproductive Biology and Endocrinology, 2: 29
Publisher:
BioMed Central
Issue Date:
2004
URI:
http://hdl.handle.net/2436/6310
DOI:
10.1186/1477-7827-2-29
Additional Links:
http://www.rbej.com/content/2/1/29
Submitted date:
2006
Type:
Article
Language:
en
ISSN:
14777827
Appears in Collections:
Diabetes, Physiology and Molecular Medicine Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSeyffarth, Gunther-
dc.contributor.authorNelson, Paul N.-
dc.contributor.authorDunmore, Simon J.-
dc.contributor.authorRodrigo, Nalinda-
dc.contributor.authorMurphy, Damian J.-
dc.contributor.authorCarson, Ray J.-
dc.date.accessioned2006-11-30T16:03:25Z-
dc.date.available2006-11-30T16:03:25Z-
dc.date.issued2004-
dc.date.submitted2006-
dc.identifier.citationReproductive Biology and Endocrinology, 2: 29en
dc.identifier.issn14777827-
dc.identifier.doi10.1186/1477-7827-2-29-
dc.identifier.urihttp://hdl.handle.net/2436/6310-
dc.description.abstractPlatelet-activating factor and nitric oxide may be involved in the initiation of human labour as inflammatory mediators. The aim of this study was to test whether platelet-activating factor and lipopolysaccharide were able to induce nitric oxide synthase expression and stimulate the production of nitric oxide in human fetal membrane explants in culture. Fetal membranes were collected from Caesarean sections at term. RNA was extracted from membranes and subjected to a qualitative RT-PCR to assess the baseline expression of iNOS. Discs of fetal membranes were cultured for 24 hours in the presence of platelet-activating factor at a dose range of 0.1 nanomolar – 1 micomolar or 1 microgram/ml lipopolysaccharide. Nitric oxide production was measured via nitrite ions in the culture medium and mRNA for iNOS was detected by RT-PCR. Culturing the membrane discs in medium containing serum induced nitric oxide synthase expression and platelet-activating factor significantly stimulated the production of nitric oxide under these conditions. When cultured without serum inducible nitric oxide synthase expression was induced by lipopolysaccharide, but not by platelet-activating factor. Platelet-activating factor may have a role in the initiation of labour, at term or preterm, via the increased local production of nitric oxide as an inflammatory mediator. In this model of intrauterine infection, lipopolysaccharide was found to induce iNOS expression by fetal membranes, and this mechanism could be involved in preterm labour.en
dc.format.extent607691 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.rbej.com/content/2/1/29en
dc.subjectLipopolysaccharideen
dc.subjectHuman fetal membranesen
dc.subjectNitric oxideen
dc.titleLipopolysaccharide induces nitric oxide synthase expression and platelet-activating factor increases nitric oxide production in human fetal membranes in cultureen
dc.typeArticleen
dc.format.digYES-
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