Electrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources

2.50
Hdl Handle:
http://hdl.handle.net/2436/620674
Title:
Electrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources
Authors:
Irorere, Victor U.; Bagheriasl, Soroosh; Blevins, Mark ( 0000-0002-9568-1624 ) ; Kwiecień, Iwona; Stamboulis, Artemis ( 0000-0002-8366-590X ) ; Radecka, Iza ( 0000-0003-3257-8803 )
Abstract:
The properties of PHB may be affected by the carbon source used in its production and this may affect nanofibres made from this polymer by electrospinning. In this study, P(3-HB) was produced from glucose, rapeseed oil, and olive oil by Ralstonia eutropha H16. Cell growth and polymer production were higher in olive or rapeseed oil supplemented media compared to glucose supplemented media. FT-IR, 1 H-, 13 C-NMR, and ESI/MS n confirmed that the synthesized polymers were P(3-HB). SEM micrograph showed the formation of nanofibres from P(3-HB) samples with the fibre diameters dependent on the source of the carbon used in polymer synthesis and the concentration of the polymer in the electrospinning solution. GPC showed that P(3-HB) from glucose (G-PHB) had a higher molecular weight ( 7.35 × 10 5  gmol −1 ) compared to P(3-HB) from rapeseed (R-PHB) and olive (O-PHB) oil. Differential scanning calorimetry (DSC) showed that the crystallinity of the electrospun polymers reduces with decreasing polymer concentration with R-PHB having lower crystallinity at all concentrations used. These observation shows that more PHB yield can be obtained using either rapeseed or olive oil compared to glucose with glucose producing polymers of higher molecular weight. It also show that electrospinning could be used to reduce the crystallinity of PHB fibres.
Citation:
Electrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources 2014, 2014:1 International Journal of Polymer Science
Journal:
International Journal of Polymer Science
Issue Date:
2014
URI:
http://hdl.handle.net/2436/620674
DOI:
10.1155/2014/705359
Additional Links:
http://www.hindawi.com/journals/ijps/2014/705359/
Type:
Article
ISSN:
1687-9422; 1687-9430
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorIrorere, Victor U.en
dc.contributor.authorBagheriasl, Sorooshen
dc.contributor.authorBlevins, Marken
dc.contributor.authorKwiecień, Iwonaen
dc.contributor.authorStamboulis, Artemisen
dc.contributor.authorRadecka, Izaen
dc.date.accessioned2017-09-20T10:25:54Z-
dc.date.available2017-09-20T10:25:54Z-
dc.date.issued2014-
dc.identifier.citationElectrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources 2014, 2014:1 International Journal of Polymer Scienceen
dc.identifier.issn1687-9422-
dc.identifier.issn1687-9430-
dc.identifier.doi10.1155/2014/705359-
dc.identifier.urihttp://hdl.handle.net/2436/620674-
dc.description.abstractThe properties of PHB may be affected by the carbon source used in its production and this may affect nanofibres made from this polymer by electrospinning. In this study, P(3-HB) was produced from glucose, rapeseed oil, and olive oil by Ralstonia eutropha H16. Cell growth and polymer production were higher in olive or rapeseed oil supplemented media compared to glucose supplemented media. FT-IR, 1 H-, 13 C-NMR, and ESI/MS n confirmed that the synthesized polymers were P(3-HB). SEM micrograph showed the formation of nanofibres from P(3-HB) samples with the fibre diameters dependent on the source of the carbon used in polymer synthesis and the concentration of the polymer in the electrospinning solution. GPC showed that P(3-HB) from glucose (G-PHB) had a higher molecular weight ( 7.35 × 10 5  gmol −1 ) compared to P(3-HB) from rapeseed (R-PHB) and olive (O-PHB) oil. Differential scanning calorimetry (DSC) showed that the crystallinity of the electrospun polymers reduces with decreasing polymer concentration with R-PHB having lower crystallinity at all concentrations used. These observation shows that more PHB yield can be obtained using either rapeseed or olive oil compared to glucose with glucose producing polymers of higher molecular weight. It also show that electrospinning could be used to reduce the crystallinity of PHB fibres.en
dc.relation.urlhttp://www.hindawi.com/journals/ijps/2014/705359/en
dc.rightsArchived with thanks to International Journal of Polymer Scienceen
dc.subjectPolyhydroxybutyrateen
dc.subjectelectrospun nanofibreen
dc.subjectRalstonia eutrophaen
dc.titleElectrospun Fibres of Polyhydroxybutyrate Synthesized by Ralstonia eutropha from Different Carbon Sources-
dc.typeArticleen
dc.identifier.journalInternational Journal of Polymer Scienceen
dc.contributor.institutionFaculty of Science and Engineering, University of Wolverhampton, Wulfruna Street, Wolverhampton WV1 1SB, UK-
dc.contributor.institutionBiomaterials Group, School of Metallurgy and Materials, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK-
dc.contributor.institutionBiomaterials Group, School of Metallurgy and Materials, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK-
dc.contributor.institutionCentre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Skłodowska Street, 41-819 Zabrze, Poland-
dc.contributor.institutionBiomaterials Group, School of Metallurgy and Materials, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK-
dc.contributor.institutionFaculty of Science and Engineering, University of Wolverhampton, Wulfruna Street, Wolverhampton WV1 1SB, UK-
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