Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production

2.50
Hdl Handle:
http://hdl.handle.net/2436/620288
Title:
Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production
Authors:
Radecka, Iza ( 0000-0003-3257-8803 ) ; Irorere, Victor; Jiang, Guozhan; Hill, David; Williams, Craig ( 0000-0001-6684-9283 ) ; Adamus, Grazyna; Kwiecień, Michal; Marek, Adam; Zawadiak, Jan; Johnston, Brian; Kowalczuk, Marek
Abstract:
We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerateand 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterizationwhen the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating unitswas very low.
Citation:
Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production 2016, 9 (5):367 Materials
Publisher:
MDPI
Journal:
Materials
Issue Date:
13-May-2016
URI:
http://hdl.handle.net/2436/620288
DOI:
10.3390/ma9050367
Additional Links:
http://www.mdpi.com/1996-1944/9/5/367
Type:
Article
Language:
en
ISSN:
1996-1944
Sponsors:
The Research Investment Fund, University of Wolverhampton (Wolverhampton, UK), the Centre of Polymer and CarbonMaterials, PAS, Poland
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorRadecka, Izaen
dc.contributor.authorIrorere, Victoren
dc.contributor.authorJiang, Guozhanen
dc.contributor.authorHill, Daviden
dc.contributor.authorWilliams, Craigen
dc.contributor.authorAdamus, Grazynaen
dc.contributor.authorKwiecień, Michalen
dc.contributor.authorMarek, Adamen
dc.contributor.authorZawadiak, Janen
dc.contributor.authorJohnston, Brianen
dc.contributor.authorKowalczuk, Mareken
dc.date.accessioned2016-11-18T10:44:39Z-
dc.date.available2016-11-18T10:44:39Z-
dc.date.issued2016-05-13-
dc.identifier.citationOxidized Polyethylene Wax as a Potential Carbon Source for PHA Production 2016, 9 (5):367 Materialsen
dc.identifier.issn1996-1944en
dc.identifier.doi10.3390/ma9050367-
dc.identifier.urihttp://hdl.handle.net/2436/620288-
dc.description.abstractWe report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerateand 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterizationwhen the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating unitswas very low.en
dc.description.sponsorshipThe Research Investment Fund, University of Wolverhampton (Wolverhampton, UK), the Centre of Polymer and CarbonMaterials, PAS, Polanden
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urlhttp://www.mdpi.com/1996-1944/9/5/367en
dc.rightsArchived with thanks to Materialsen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectpolyhdyroxyalkanoatesen
dc.subjectoxidized PE waxen
dc.subjectmedium chain length PHA (mcl-PHA)en
dc.subjectRalstonia eutropha H16en
dc.subjectmass spectrometryen
dc.titleOxidized Polyethylene Wax as a Potential Carbon Source for PHA Productionen
dc.typeArticleen
dc.identifier.journalMaterialsen
dc.date.accepted2016-05-
rioxxterms.funderThe Research Investment Fund, University of Wolverhampton (Wolverhampton, UK), the Centre of Polymer and CarbonMaterials, PAS, Polanden
rioxxterms.identifier.projectR20472-1en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttps://creativecommons.org/CC BY-NC-ND 4.0en
rioxxterms.licenseref.startdate2016-11-18en
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