The Characterisation of an Adrenergic Signalling System Involved in the Encystment of the Ocular Pathogen Acanthamoeba spp.

2.50
Hdl Handle:
http://hdl.handle.net/2436/611713
Title:
The Characterisation of an Adrenergic Signalling System Involved in the Encystment of the Ocular Pathogen Acanthamoeba spp.
Authors:
Heaselgrave, Wayne; Kilvington, Simon
Abstract:
The aim of this study was to identify and characterise the receptor system involved in controlling encystment in Acanthamoeba using specific agonists and antagonists and to examine whether endogenous stores of catecholamines are produced by the organism. Acanthamoeba trophozoites suspended in axenic growth medium were exposed to adrenoceptor agonists and antagonists to determine which compounds promoted or prevented encystment. Secondly, trophozoites were cultured in medium containing a catecholamine synthesis inhibitor to investigate the effect this had on natural encystment. Non-specific adrenoceptor agonists including epinephrine, isoprotenerol and the selective β1 adrenoceptor agonist dobutamine were found to cause >90% encystment of Acanthamoeba trophozoites compared to <30% with the controls. The selective β1 antagonist metoprolol was able to inhibit epinephrine mediated encystment by >55%. Cultures of Acanthamoeba with the catecholamine synthesis inhibitor α-methyl-p-tyrosine significantly reduced the level of amoebic encystment compared to controls. In conclusion Acanthamoeba appear to contain a functional adrenergic receptor system of unknown structure which is involved in initiating the encystment process that can be activated and blocked by β1 agonists and antagonists respectively. Furthermore the presence of this receptor system in Acanthamoeba indicates that topical β adrenoceptor blockers may be effective adjunct therapy by reducing the transformation of trophozoites into the highly resistant cyst stage. This article is protected by copyright. All rights reserved.
Citation:
The Characterisation of an Adrenergic Signalling System Involved in the Encystment of the Ocular Pathogen Acanthamoeba spp. 2016: J. Eukaryot. Microbiol.
Publisher:
Wiley
Journal:
The Journal of eukaryotic microbiology,
Issue Date:
24-Mar-2016
URI:
http://hdl.handle.net/2436/611713
DOI:
10.1111/jeu.12312
PubMed ID:
26941040
Type:
Article
Language:
en
ISSN:
1550-7408
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorHeaselgrave, Wayneen
dc.contributor.authorKilvington, Simonen
dc.date.accessioned2016-06-03T13:04:06Zen
dc.date.available2016-06-03T13:04:06Zen
dc.date.issued2016-03-24en
dc.identifier.citationThe Characterisation of an Adrenergic Signalling System Involved in the Encystment of the Ocular Pathogen Acanthamoeba spp. 2016: J. Eukaryot. Microbiol.en
dc.identifier.issn1550-7408en
dc.identifier.pmid26941040en
dc.identifier.doi10.1111/jeu.12312en
dc.identifier.urihttp://hdl.handle.net/2436/611713en
dc.description.abstractThe aim of this study was to identify and characterise the receptor system involved in controlling encystment in Acanthamoeba using specific agonists and antagonists and to examine whether endogenous stores of catecholamines are produced by the organism. Acanthamoeba trophozoites suspended in axenic growth medium were exposed to adrenoceptor agonists and antagonists to determine which compounds promoted or prevented encystment. Secondly, trophozoites were cultured in medium containing a catecholamine synthesis inhibitor to investigate the effect this had on natural encystment. Non-specific adrenoceptor agonists including epinephrine, isoprotenerol and the selective β1 adrenoceptor agonist dobutamine were found to cause >90% encystment of Acanthamoeba trophozoites compared to <30% with the controls. The selective β1 antagonist metoprolol was able to inhibit epinephrine mediated encystment by >55%. Cultures of Acanthamoeba with the catecholamine synthesis inhibitor α-methyl-p-tyrosine significantly reduced the level of amoebic encystment compared to controls. In conclusion Acanthamoeba appear to contain a functional adrenergic receptor system of unknown structure which is involved in initiating the encystment process that can be activated and blocked by β1 agonists and antagonists respectively. Furthermore the presence of this receptor system in Acanthamoeba indicates that topical β adrenoceptor blockers may be effective adjunct therapy by reducing the transformation of trophozoites into the highly resistant cyst stage. This article is protected by copyright. All rights reserved.en
dc.languageENGen
dc.language.isoenen
dc.publisherWileyen
dc.rightsArchived with thanks to The Journal of eukaryotic microbiologyen
dc.subjectAcanthamoebaen
dc.subjectencystmenten
dc.subjectcatecholamineen
dc.titleThe Characterisation of an Adrenergic Signalling System Involved in the Encystment of the Ocular Pathogen Acanthamoeba spp.en
dc.typeArticleen
dc.identifier.journalThe Journal of eukaryotic microbiology,en

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