Inactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma.

2.50
Hdl Handle:
http://hdl.handle.net/2436/611712
Title:
Inactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma.
Authors:
Heaselgrave, Wayne; Shama, Gilbert; Andrew, Peter W; Kong, Michael G
Abstract:
Currently there are estimated to be approximately 3.7 million contact lens wearers in the United Kingdom and 39.2 million in North America. Contact lens wear is a major risk factor for developing an infection of the cornea known as keratitis due to poor lens hygiene practices. While there is an international standard for testing disinfection methods against bacteria and fungi (ISO 14729), no such guidelines exist for the protozoan Acanthamoeba, which causes a potentially blinding keratitis most commonly seen in contact lens wearers, and as a result, many commercially available disinfecting solutions show incomplete disinfection after 6 and 24 h of exposure. Challenge test assays based on international standard ISO 14729 were used to determine the antimicrobial activity of cold atmospheric gas plasma (CAP) against Pseudomonas aeruginosa, Candida albicans, and trophozoites and cysts of Acanthamoeba polyphaga and Acanthamoeba castellanii P. aeruginosa and C. albicans were completely inactivated in 0.5 min and 2 min, respectively, and trophozoites of A. polyphaga and A. castellanii were completely inactivated in 1 min and 2 min, respectively. Furthermore, for the highly resistant cyst stage of both species, complete inactivation was achieved after 4 min of exposure to CAP. This study demonstrates that the CAP technology is highly effective against bacterial, fungal, and protozoan pathogens. The further development of this technology has enormous potential, as this approach is able to deliver the complete inactivation of ocular pathogens in minutes, in contrast to commercial multipurpose disinfecting solutions that require a minimum of 6 h.
Citation:
Inactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma. 2016, 82 (10):3143-8 Appl. Environ. Microbiol.
Publisher:
American Society for Microbiology
Journal:
Applied and environmental microbiology, May 2016 ; 82:10 3143-3148
Issue Date:
15-May-2016
URI:
http://hdl.handle.net/2436/611712
DOI:
10.1128/AEM.03863-15
PubMed ID:
26994079
Additional Links:
http://aem.asm.org/content/82/10.toc
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorHeaselgrave, Wayneen
dc.contributor.authorShama, Gilberten
dc.contributor.authorAndrew, Peter Wen
dc.contributor.authorKong, Michael Gen
dc.date.accessioned2016-06-03T12:40:28Zen
dc.date.available2016-06-03T12:40:28Zen
dc.date.issued2016-05-15en
dc.identifier.citationInactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma. 2016, 82 (10):3143-8 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336en
dc.identifier.pmid26994079en
dc.identifier.doi10.1128/AEM.03863-15en
dc.identifier.urihttp://hdl.handle.net/2436/611712en
dc.description.abstractCurrently there are estimated to be approximately 3.7 million contact lens wearers in the United Kingdom and 39.2 million in North America. Contact lens wear is a major risk factor for developing an infection of the cornea known as keratitis due to poor lens hygiene practices. While there is an international standard for testing disinfection methods against bacteria and fungi (ISO 14729), no such guidelines exist for the protozoan Acanthamoeba, which causes a potentially blinding keratitis most commonly seen in contact lens wearers, and as a result, many commercially available disinfecting solutions show incomplete disinfection after 6 and 24 h of exposure. Challenge test assays based on international standard ISO 14729 were used to determine the antimicrobial activity of cold atmospheric gas plasma (CAP) against Pseudomonas aeruginosa, Candida albicans, and trophozoites and cysts of Acanthamoeba polyphaga and Acanthamoeba castellanii P. aeruginosa and C. albicans were completely inactivated in 0.5 min and 2 min, respectively, and trophozoites of A. polyphaga and A. castellanii were completely inactivated in 1 min and 2 min, respectively. Furthermore, for the highly resistant cyst stage of both species, complete inactivation was achieved after 4 min of exposure to CAP. This study demonstrates that the CAP technology is highly effective against bacterial, fungal, and protozoan pathogens. The further development of this technology has enormous potential, as this approach is able to deliver the complete inactivation of ocular pathogens in minutes, in contrast to commercial multipurpose disinfecting solutions that require a minimum of 6 h.en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://aem.asm.org/content/82/10.tocen
dc.rightsArchived with thanks to Applied and environmental microbiologyen
dc.subjectAcanthamoebaen
dc.subjectplasmaen
dc.subjectdisinfectionen
dc.titleInactivation of Acanthamoeba spp. and Other Ocular Pathogens by Application of Cold Atmospheric Gas Plasma.en
dc.typeArticleen
dc.identifier.journalApplied and environmental microbiology, May 2016 ; 82:10 3143-3148en

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