An investigation of self-incompatibility within the genus Restrepia

2.50
Hdl Handle:
http://hdl.handle.net/2436/603642
Title:
An investigation of self-incompatibility within the genus Restrepia
Authors:
Millner, H. J.; McCrea, A. R.; Baldwin, T. C.
Abstract:
• Premise of the study: The genus Restrepia (Orchidaceae) is indigenous to montane rain forests of Central and South America. Recently, as habitat has fragmented and wild populations dwindled, the chances for successful cross-pollination within the genus have been reduced. Since cultivated species of Restrepia have been vegetatively propagated, they remain genetically close to those in the wild, making ex situ collections of the genus useful model populations for investigating breeding systems. Restrepia are found in clade B of the Pleurothallidinae, the only clade in which self-incompatibility (SI) has not yet been confirmed. In the current study, private collections of Restrepia were used to study the operation of SI within the genus to assist future ex situ conservation of this and related genera. • Methods: A variety of self-pollination, intraspecific, and interspecific crosses were performed across the genus, and pollen tube growth was studied. • Key results: Individual species exhibited varying degrees of SI. Self-pollinations performed across 26 species in the genus produced few viable seeds, with the exception of R. aberrans. Viable “filled” seeds with embryos were shown to require an intraspecific cross. Primary hybrids between species produced >90% seeds with embryos that germinated well. • Conclusions: The type of SI operating within the genus was considered to be best explained by gametophytic self-incompatibility (GSI) with interspecific variation in its phenotypic expression. The implications of these findings are discussed in relation to SI in the Pleurothallidinae and conservation strategies for Restrepia and related genera.
Citation:
An investigation of self-incompatibility within the genus Restrepia 2015, 102 (3):487 American Journal of Botany
Journal:
American Journal of Botany
Issue Date:
13-Mar-2015
URI:
http://hdl.handle.net/2436/603642
DOI:
10.3732/ajb.1400555
Additional Links:
http://www.amjbot.org/cgi/doi/10.3732/ajb.1400555
Type:
Article
ISSN:
0002-9122; 1537-2197
Appears in Collections:
FSE

Full metadata record

DC FieldValue Language
dc.contributor.authorMillner, H. J.en
dc.contributor.authorMcCrea, A. R.en
dc.contributor.authorBaldwin, T. C.en
dc.date.accessioned2016-03-24T15:32:03Zen
dc.date.available2016-03-24T15:32:03Zen
dc.date.issued2015-03-13en
dc.identifier.citationAn investigation of self-incompatibility within the genus Restrepia 2015, 102 (3):487 American Journal of Botanyen
dc.identifier.issn0002-9122en
dc.identifier.issn1537-2197en
dc.identifier.doi10.3732/ajb.1400555en
dc.identifier.urihttp://hdl.handle.net/2436/603642en
dc.description.abstract• Premise of the study: The genus Restrepia (Orchidaceae) is indigenous to montane rain forests of Central and South America. Recently, as habitat has fragmented and wild populations dwindled, the chances for successful cross-pollination within the genus have been reduced. Since cultivated species of Restrepia have been vegetatively propagated, they remain genetically close to those in the wild, making ex situ collections of the genus useful model populations for investigating breeding systems. Restrepia are found in clade B of the Pleurothallidinae, the only clade in which self-incompatibility (SI) has not yet been confirmed. In the current study, private collections of Restrepia were used to study the operation of SI within the genus to assist future ex situ conservation of this and related genera. • Methods: A variety of self-pollination, intraspecific, and interspecific crosses were performed across the genus, and pollen tube growth was studied. • Key results: Individual species exhibited varying degrees of SI. Self-pollinations performed across 26 species in the genus produced few viable seeds, with the exception of R. aberrans. Viable “filled” seeds with embryos were shown to require an intraspecific cross. Primary hybrids between species produced >90% seeds with embryos that germinated well. • Conclusions: The type of SI operating within the genus was considered to be best explained by gametophytic self-incompatibility (GSI) with interspecific variation in its phenotypic expression. The implications of these findings are discussed in relation to SI in the Pleurothallidinae and conservation strategies for Restrepia and related genera.en
dc.relation.urlhttp://www.amjbot.org/cgi/doi/10.3732/ajb.1400555en
dc.rightsArchived with thanks to American Journal of Botanyen
dc.subjectClade Ben
dc.subjectex situ conservationen
dc.subjectOrchidaceaeen
dc.subjectPleurothallidinaeen
dc.subjectRestrepiaen
dc.subjectself-incompatibilityen
dc.titleAn investigation of self-incompatibility within the genus Restrepiaen
dc.typeArticleen
dc.identifier.journalAmerican Journal of Botanyen
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