Association of interleukin-6 (IL-6)-174G/C gene polymorphism with cardiovascular disease in patients with rheumatoid arthritis: The role of obesity and smoking

2.50
Hdl Handle:
http://hdl.handle.net/2436/39979
Title:
Association of interleukin-6 (IL-6)-174G/C gene polymorphism with cardiovascular disease in patients with rheumatoid arthritis: The role of obesity and smoking
Authors:
Panoulas, Vasileios F.; Stavropoulos-Kalinoglou, Antonios; Metsios, Giorgos S.; Smith, Jacqueline P.; Milionis, Haralampos J.; Douglas, Karen M. J.; Nightingale, Peter; Kitas, George D.
Abstract:
Abstract Background Cardiovascular morbidity and mortality are increased in rheumatoid arthritis (RA). Interleukin-6 (IL-6) is high in RA and, together with smoking and obesity, an important contributor to the development of cardiovascular disease (CVD). The present study examined the potential association of IL-6-174 G/C polymorphism, together with obesity and smoking, with the presence of CVD in RA patients. Methods and results DNA samples were collected from 383 RA patients (who also had extensive clinical and laboratory evaluations). IL-6-174 G/C was identified using real time PCR and melting curve analysis. Serum IL-6 levels were measured in a subgroup of 135 RA patients to examine the functionality of the polymorphism. Carriers of the IL6-174C-allele demonstrated increased prevalence of CVD (26.2% vs. 17.0%, p = 0.041). There was a significant association with CVD, even after adjustment for traditional CVD risk factors (OR = 1.92, 95%CI: 1.03 to 3.58, p = 0.041). IL-6 levels were significantly increased in C-allele carriers [14.02 (3.21–38.81) vs. 4.48 (2.25–16.5), p = 0.028]. No significant interactions were observed between adiposity and IL6-174G/C genotypes. There was only a trend for an interaction between ever smoking and IL6 C-allele carriers on CVD. Conclusion The IL-6-174C-allele may associate with CVD in RA patients and possibly exerts its effect via increased inflammation. This finding, if confirmed in future studies, may be used as a part of a genetic screening tool for RA patients at high CVD risk.
Citation:
Atherosclerosis, [IN PRESS]
Publisher:
Elsevier Ltd.
Journal:
Atherosclerosis
Issue Date:
2008
URI:
http://hdl.handle.net/2436/39979
DOI:
10.1016/j.atherosclerosis.2008.08.036
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T12-4TCR1V1-5&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=f3e48d9e6fceba0191df20d05ba695b6
Type:
Article
Language:
en
ISSN:
00219150
Appears in Collections:
Sport, Exercise and Health Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorPanoulas, Vasileios F.-
dc.contributor.authorStavropoulos-Kalinoglou, Antonios-
dc.contributor.authorMetsios, Giorgos S.-
dc.contributor.authorSmith, Jacqueline P.-
dc.contributor.authorMilionis, Haralampos J.-
dc.contributor.authorDouglas, Karen M. J.-
dc.contributor.authorNightingale, Peter-
dc.contributor.authorKitas, George D.-
dc.date.accessioned2008-10-30T22:41:15Z-
dc.date.available2008-10-30T22:41:15Z-
dc.date.issued2008-
dc.identifier.citationAtherosclerosis, [IN PRESS]en
dc.identifier.issn00219150-
dc.identifier.doi10.1016/j.atherosclerosis.2008.08.036-
dc.identifier.urihttp://hdl.handle.net/2436/39979-
dc.description.abstractAbstract Background Cardiovascular morbidity and mortality are increased in rheumatoid arthritis (RA). Interleukin-6 (IL-6) is high in RA and, together with smoking and obesity, an important contributor to the development of cardiovascular disease (CVD). The present study examined the potential association of IL-6-174 G/C polymorphism, together with obesity and smoking, with the presence of CVD in RA patients. Methods and results DNA samples were collected from 383 RA patients (who also had extensive clinical and laboratory evaluations). IL-6-174 G/C was identified using real time PCR and melting curve analysis. Serum IL-6 levels were measured in a subgroup of 135 RA patients to examine the functionality of the polymorphism. Carriers of the IL6-174C-allele demonstrated increased prevalence of CVD (26.2% vs. 17.0%, p = 0.041). There was a significant association with CVD, even after adjustment for traditional CVD risk factors (OR = 1.92, 95%CI: 1.03 to 3.58, p = 0.041). IL-6 levels were significantly increased in C-allele carriers [14.02 (3.21–38.81) vs. 4.48 (2.25–16.5), p = 0.028]. No significant interactions were observed between adiposity and IL6-174G/C genotypes. There was only a trend for an interaction between ever smoking and IL6 C-allele carriers on CVD. Conclusion The IL-6-174C-allele may associate with CVD in RA patients and possibly exerts its effect via increased inflammation. This finding, if confirmed in future studies, may be used as a part of a genetic screening tool for RA patients at high CVD risk.en
dc.language.isoenen
dc.publisherElsevier Ltd.en
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T12-4TCR1V1-5&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=f3e48d9e6fceba0191df20d05ba695b6-
dc.subjectRheumatoid Arthritisen
dc.subjectCardiovascular Diseaseen
dc.subjectInterleukin 6 geneen
dc.subjectIL6-174G/C polymorphismen
dc.subjectSmokingen
dc.subjectObesityen
dc.subjectArthritis, Rheumatoid-
dc.titleAssociation of interleukin-6 (IL-6)-174G/C gene polymorphism with cardiovascular disease in patients with rheumatoid arthritis: The role of obesity and smokingen
dc.typeArticleen
dc.identifier.journalAtherosclerosisen
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