University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > Mitoparan and target-selective chimeric analogues: membrane translocation and intracellular redistribution induces mitochondrial apoptosis.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/30272
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Mitoparan and target-selective chimeric analogues: membrane translocation and intracellular redistribution induces mitochondrial apoptosis.
Authors: Jones, Sarah
Martel, Cecile
Belzacq-Casagrande, Anne-Sophie
Brenner, Catherine
Howl, John D.
Citation: Biochimica et Biophysica Acta - Molecular Cell Research, 1783(5): 849-863
Publisher: Amsterdam: Elsevier
Journal: Biochimica et Biophysica Acta - Molecular Cell Research
Issue Date: 2008
URI: http://hdl.handle.net/2436/30272
DOI: 10.1016/j.bbamcr.2008.01.009
PubMed ID: 18267123
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T20-4RP0MF4-1&_user=10&_coverDate=05%2F31%2F2008&_rdoc=17&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234904%232008%23982169994%23686190%23FLA%23display%23Volume)&_cdi=4904&_sort=d&_docanchor=&_ct=26&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=78ecb320a945265feb7eb85cf01981e6
Abstract: Mastoparan, and structurally-related amphipathic peptides, may induce cell death by augmentation of necrotic and/or apoptotic pathways. To more precisely delineate cytotoxic mechanisms, we determined that [Lys(5,8)Aib(10)]mastoparan (mitoparan) specifically induces apoptosis of U373MG and ECV304 cells, as demonstrated by endonuclease and caspase-3 activation and phosphatidylserine translocation. Live cell imaging confirmed that, following translocation of the plasma membrane, mitoparan specifically co-localizes with mitochondria. Complementary studies indicated that mitoparan induces swelling and permeabilization of isolated mitochondria, through cooperation with a protein of the permeability transition pore complex VDAC, leading to the release of the apoptogenic factor, cytochrome c. N-terminal acylation of mitoparan facilitated the synthesis of chimeric peptides that incorporated target-specific address motifs including an integrin-specific RGD sequence and a Fas ligand mimetic. Significantly, these sychnologically-organised peptides demonstrated further enhanced cytotoxic potencies. We conclude that the cell penetrant, mitochondriotoxic and apoptogenic properties of mitoparan, and its chimeric analogues, offer new insights to the study and therapeutic induction of apoptosis.
Type: Article
Language: en
Keywords: Peptides
Chimerism
Mastoparan
Mitochondria
Apoptosis
Peptide Mastoparan
Cell Penetrating Peptides (CPP)
Apoptosis
CPP
Cytotoxicity
Molecular Biology
ISSN: 0006-3002
Appears in Collections: Molecular Pharmacology Research Group

Files in This Item:

There are no files associated with this item.



Related articles on PubMed
bullet
bullet
Targeted Vpr-derived peptides reach mitochondria to induce apoptosis of alphaVbeta3-expressing endothelial cells.
Borgne-Sanchez A, Dupont S, Langonné A, Baux L, Lecoeur H, Chauvier D, Lassalle M, Déas O, Brière JJ, Brabant M, Roux P, Péchoux C, Briand JP, Hoebeke J, Deniaud A, Brenner C, Rustin P, Edelman L, Rebouillat D, Jacotot E
2007 Mar
bullet
Inflammation and apoptosis induced by mastoparan Polybia-MPII on skeletal muscle.
Rocha T, de Barros LL, Fontana K, de Souza BM, Palma MS, da Cruz-Höfling MA
2010 Jun 15
bullet
Cobalt induces oxidative stress in isolated liver mitochondria responsible for permeability transition and intrinsic apoptosis in hepatocyte primary cultures.
Battaglia V, Compagnone A, Bandino A, Bragadin M, Rossi CA, Zanetti F, Colombatto S, Grillo MA, Toninello A
2009 Mar
bullet
See all 87 articles

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies