University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Immunology Research Group  > Generation and characterization of monoclonal antibodies to the neural crest.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/30255
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Generation and characterization of monoclonal antibodies to the neural crest.
Authors: Shakil, T.
Richardson, M. K.
Waldron, E.E.
Conde, Gillian
Wood, S.
Bland, Y.
Reynolds, Gary
Murray, Paul G.
Nelson, Paul N.
Citation: Hybridoma, 20(3): 199-203
Publisher: New Rochelle (NY): Mary Ann Liebert, Inc.
Journal: Hybridoma
Issue Date: 2001
URI: http://hdl.handle.net/2436/30255
DOI: 10.1089/027245701750293538
PubMed ID: 11461669
Additional Links: http://www.liebertonline.com/doi/abs/10.1089/027245701750293538?journalCode=hyb.1
http://direct.bl.uk/bld/PlaceOrder.do?UIN=098727917&ETOC=RN&from=searchengine
Abstract: The generation of monoclonal antibodies (MAbs) specific for quail neural crest may provide valuable tools for studying the differentiation of embryonic precursor cells. Unfortunately, relatively few antibodies are available because of the difficulty in obtaining sufficient cells for in vivo immunization strategies. We have overcome this problem by using intrasplenic immunization with formaldehyde-fixed cells harvested from neural crest cultures. In addition, booster injections of cultured whole-embryo cells were administered intraperitoneally. Following two fusions, a total of 18 hybridomas were generated with antibody reactivity to the cytoplasm of neural crest cells. Furthermore, 32 were reactive against both somite (a noncrest mesodermal control) and crest cultures, whilst 15 were not reactive. Out of those hybridomas reactive with neural crest, six designated 160D, 164D, OE, 12E, 120E and 124E were further characterized. Interestingly MAb supernatants OE, 12E, 120E, and 124E exhibited reactivity against some but not all neural crest cells suggesting that they might recognise subpopulations. Immunoglobulin isotyping of supernatants revealed that 4 (160D, 164D, OE, and 120E) were IgM and 2 (12E and 124E) were IgG(2b). On assessing their reactivity against human tissue sections, all six hybridoma supernatants cross-reacted with neuroendocrine cells within appendix, colon and rectum. These MAbs could provide novel reagents for the understanding of neural crest development.
Type: Article
Language: en
Keywords: Monoclonal antibodies
Hybridomas
Oncology
Molecular Biology
MeSH: Animals
Antibodies, Monoclonal
Antibody Specificity
Humans
Mice
Neural Crest
ISSN: 0272-457X
Appears in Collections: Molecular Immunology Research Group

Files in This Item:

There are no files associated with this item.



Related articles on PubMed
bullet
bullet
bullet
bullet
bullet
See all 94 articles

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies