2.50
Hdl Handle:
http://hdl.handle.net/2436/30255
Title:
Generation and characterization of monoclonal antibodies to the neural crest.
Authors:
Shakil, T.; Richardson, M. K.; Waldron, E.E.; Conde, Gillian; Wood, S.; Bland, Y.; Reynolds, Gary; Murray, Paul G.; Nelson, Paul N.
Abstract:
The generation of monoclonal antibodies (MAbs) specific for quail neural crest may provide valuable tools for studying the differentiation of embryonic precursor cells. Unfortunately, relatively few antibodies are available because of the difficulty in obtaining sufficient cells for in vivo immunization strategies. We have overcome this problem by using intrasplenic immunization with formaldehyde-fixed cells harvested from neural crest cultures. In addition, booster injections of cultured whole-embryo cells were administered intraperitoneally. Following two fusions, a total of 18 hybridomas were generated with antibody reactivity to the cytoplasm of neural crest cells. Furthermore, 32 were reactive against both somite (a noncrest mesodermal control) and crest cultures, whilst 15 were not reactive. Out of those hybridomas reactive with neural crest, six designated 160D, 164D, OE, 12E, 120E and 124E were further characterized. Interestingly MAb supernatants OE, 12E, 120E, and 124E exhibited reactivity against some but not all neural crest cells suggesting that they might recognise subpopulations. Immunoglobulin isotyping of supernatants revealed that 4 (160D, 164D, OE, and 120E) were IgM and 2 (12E and 124E) were IgG(2b). On assessing their reactivity against human tissue sections, all six hybridoma supernatants cross-reacted with neuroendocrine cells within appendix, colon and rectum. These MAbs could provide novel reagents for the understanding of neural crest development.
Citation:
Hybridoma, 20(3): 199-203
Publisher:
New Rochelle (NY): Mary Ann Liebert, Inc.
Journal:
Hybridoma
Issue Date:
2001
URI:
http://hdl.handle.net/2436/30255
DOI:
10.1089/027245701750293538
PubMed ID:
11461669
Additional Links:
http://www.liebertonline.com/doi/abs/10.1089/027245701750293538?journalCode=hyb.1; http://direct.bl.uk/bld/PlaceOrder.do?UIN=098727917&ETOC=RN&from=searchengine
Type:
Article
Language:
en
ISSN:
0272-457X
Appears in Collections:
Molecular Immunology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorShakil, T.-
dc.contributor.authorRichardson, M. K.-
dc.contributor.authorWaldron, E.E.-
dc.contributor.authorConde, Gillian-
dc.contributor.authorWood, S.-
dc.contributor.authorBland, Y.-
dc.contributor.authorReynolds, Gary-
dc.contributor.authorMurray, Paul G.-
dc.contributor.authorNelson, Paul N.-
dc.date.accessioned2008-06-20T10:39:53Z-
dc.date.available2008-06-20T10:39:53Z-
dc.date.issued2001-
dc.identifier.citationHybridoma, 20(3): 199-203en
dc.identifier.issn0272-457X-
dc.identifier.pmid11461669-
dc.identifier.doi10.1089/027245701750293538-
dc.identifier.urihttp://hdl.handle.net/2436/30255-
dc.description.abstractThe generation of monoclonal antibodies (MAbs) specific for quail neural crest may provide valuable tools for studying the differentiation of embryonic precursor cells. Unfortunately, relatively few antibodies are available because of the difficulty in obtaining sufficient cells for in vivo immunization strategies. We have overcome this problem by using intrasplenic immunization with formaldehyde-fixed cells harvested from neural crest cultures. In addition, booster injections of cultured whole-embryo cells were administered intraperitoneally. Following two fusions, a total of 18 hybridomas were generated with antibody reactivity to the cytoplasm of neural crest cells. Furthermore, 32 were reactive against both somite (a noncrest mesodermal control) and crest cultures, whilst 15 were not reactive. Out of those hybridomas reactive with neural crest, six designated 160D, 164D, OE, 12E, 120E and 124E were further characterized. Interestingly MAb supernatants OE, 12E, 120E, and 124E exhibited reactivity against some but not all neural crest cells suggesting that they might recognise subpopulations. Immunoglobulin isotyping of supernatants revealed that 4 (160D, 164D, OE, and 120E) were IgM and 2 (12E and 124E) were IgG(2b). On assessing their reactivity against human tissue sections, all six hybridoma supernatants cross-reacted with neuroendocrine cells within appendix, colon and rectum. These MAbs could provide novel reagents for the understanding of neural crest development.en
dc.language.isoenen
dc.publisherNew Rochelle (NY): Mary Ann Liebert, Inc.en
dc.relation.urlhttp://www.liebertonline.com/doi/abs/10.1089/027245701750293538?journalCode=hyb.1en
dc.relation.urlhttp://direct.bl.uk/bld/PlaceOrder.do?UIN=098727917&ETOC=RN&from=searchengine-
dc.subjectMonoclonal antibodiesen
dc.subjectHybridomasen
dc.subjectOncologyen
dc.subjectMolecular Biologyen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Monoclonalen
dc.subject.meshAntibody Specificityen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshNeural Cresten
dc.titleGeneration and characterization of monoclonal antibodies to the neural crest.en
dc.typeArticleen
dc.identifier.journalHybridomaen
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