2.50
Hdl Handle:
http://hdl.handle.net/2436/30203
Title:
Demystified...recombinant antibodies.
Authors:
Smith, K.A.; Nelson, Paul N.; Warren, Phil; Astley, S.J.; Murray, Paul G.; Greenman, J.
Abstract:
Recombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanized antibodies is given, together with details of the generation of antibody fragments--for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.
Citation:
Journal of Clinical Pathology, 57(9): 912-917
Publisher:
BMJ Publishing Group Ltd.
Journal:
Journal of Clinical Pathology
Issue Date:
2004
URI:
http://hdl.handle.net/2436/30203
DOI:
10.1136/jcp.2003.014407
PubMed ID:
15333649
Additional Links:
http://jcp.bmj.com/cgi/content/abstract/57/9/912
Type:
Article
Language:
en
ISSN:
0021-9746
Appears in Collections:
Molecular Immunology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSmith, K.A.-
dc.contributor.authorNelson, Paul N.-
dc.contributor.authorWarren, Phil-
dc.contributor.authorAstley, S.J.-
dc.contributor.authorMurray, Paul G.-
dc.contributor.authorGreenman, J.-
dc.date.accessioned2008-06-19T15:21:00Z-
dc.date.available2008-06-19T15:21:00Z-
dc.date.issued2004-
dc.identifier.citationJournal of Clinical Pathology, 57(9): 912-917en
dc.identifier.issn0021-9746-
dc.identifier.pmid15333649-
dc.identifier.doi10.1136/jcp.2003.014407-
dc.identifier.urihttp://hdl.handle.net/2436/30203-
dc.description.abstractRecombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanized antibodies is given, together with details of the generation of antibody fragments--for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.en
dc.language.isoenen
dc.publisherBMJ Publishing Group Ltd.en
dc.relation.urlhttp://jcp.bmj.com/cgi/content/abstract/57/9/912en
dc.subjectPhage displayen
dc.subjectMonoclonal antibodiesen
dc.subjectMolecular Biologyen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Monoclonalen
dc.subject.meshFlow Cytometryen
dc.subject.meshHumansen
dc.subject.meshImmunoglobulin Fragmentsen
dc.subject.meshImmunohistochemistryen
dc.subject.meshPeptide Libraryen
dc.subject.meshRecombinant Proteinsen
dc.titleDemystified...recombinant antibodies.en
dc.typeArticleen
dc.identifier.journalJournal of Clinical Pathologyen

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