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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Immunology Research Group  > Characterization of anti-myosin monoclonal antibodies.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/30202
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Title: Characterization of anti-myosin monoclonal antibodies.
Authors: Nelson, Paul N.
Astley, S.J.
Roden, Denise A.
Waldron, E.E.
Baig, K.M.
Caforio, A.L.
Koutedakis, Yiannis
Perera, Shantha
Spry, C.
Citation: Hybridoma, 24(6): 314-318
Publisher: New Rochelle (NY): Mary Ann Liebert, Inc.
Journal: Hybridoma
Issue Date: 2005
URI: http://hdl.handle.net/2436/30202
DOI: 10.1089/hyb.2005.24.314
PubMed ID: 16332199
Additional Links: http://www.liebertonline.com/doi/abs/10.1089/hyb.2005.24.314
http://direct.bl.uk/bld/PlaceOrder.do?UIN=178812929&ETOC=RN&from=searchengine
Abstract: The characterization of monoclonal antibodies (MAbs) with regard to reactivity and specificity is important for the successful application as a molecular probe and/or diagnostic reagent. Furthermore, it is recognized that some monoclonal reagents perform well in some assay systems but not others. In this study, the reactivity profiles of two anti-myosin MAbs (H1 and DH2, raised against human cardiac myosin) were evaluated in enzyme-linked immunosorbent assay (ELISA), slot-blotting, and immunocytochemistry. Both antibodies performed well in slot-blotting against myosin heavy chain preparations from cardiac and skeletal muscle and from non-human sources. In general, MAb H1 demonstrated strong to moderate reactivity in all assay systems, whilst MAb DH2 faired poorly in ELISA. MAb H1 also showed reactivity to synthetic peptides of myosin, one of which possessed a motif (ERRDA, single amino acid code) that was found in other human and nonhuman myosin protein sequences that could explain its cross-reactive profile. Intriguingly, this motif was found on viral and other pathogenic agents associated with myocarditis. Hence, it is speculated that this region could give some credence to the mechanism of molecular mimicry associated with some cardiac diseases. Overall, MAb H1 may serve as a useful probe of myosin structure.
Type: Article
Language: en
Keywords: Monoclonal antibodies
Molecular Biology
ELISA
Blotting, Slot
Myosin
Heart
MeSH: Amino Acid Motifs
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antibody Specificity
Cardiac Myosins
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Humans
Hybridomas
Immunohistochemistry
Molecular Sequence Data
ISSN: 1554-0014
Appears in Collections: Molecular Immunology Research Group

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