Correlation of copy number aberrations with clinico-pathological criteria in paediatric glial tumours

2.50
Hdl Handle:
http://hdl.handle.net/2436/29985
Title:
Correlation of copy number aberrations with clinico-pathological criteria in paediatric glial tumours
Authors:
Ward, Samantha; Hayward, Richard; Harkness, William; Phipps, Kim; Thompson, Dominic; Harding, Brian; Wilkins, Peter; Darling, John L.; Thomas, David G.; Warr, Tracy
Other Titles:
In: Tenth International Symposium on Pediatric Neuro-Oncology, June 9–12, 2002, London, England. No.194
Abstract:
Glial cell tumours represent the largest group of brain tumours in childhood and include astrocytoma (WHO grades I-IV) and ependymoma (WHO grade II-III). However, little is known about the pathogenesis of these tumours. We have used comparative genomic hybridisation (CGH) to investigate the genetic alterations in 128 tumours from children and young adults (< 30 years of age) comprising 52 ependymoma, including 40 samples that have previously been reported (44 grade II and 8 grade III) and 76 astrocytoma (consisting of 34 grade I, 17 grade II, 7 grade III, and 18 grade IV). Genetic alterations were compared to clinicopathological data such as histology, tumour recurrence, and survival in order to identify potential prognostic markers. In ependymoma, 39% of the tumours had no detectable copy number aberrations (CNAs). In the remaining tumours, the most common regions of gain were 4q (29%), 6q (21%), 1q (17%), and 2q (15%). The most common regions of loss were 22 (29%), 16p (17%), 17p (13%), and 20q (13%). Three regions of high copy number amplification were observed in 3 tumours at 1q24-31 (3 cases), 8q21-23 (3 cases), and 9p (1 case). There was no association between any CNA and histology, tumour recurrence, or length of survival. In contrast, in the astrocytoma group there was a clear association between histology and the presence of CNAs. The pattern of genetic alterations became increasingly complex with tumour grade, and grade IV tumours were more likely to have CNAs than lower grade tumours (p = 0.0502). Overall, the most frequent alterations observed in astrocytoma were gain of 4q (11%), loss 16p (10.5%), and loss 17p (10.5%). However, several CNAs were seen predominantly in grade IV tumours (gain 1q, 2q, 4q, and 5q). Fourteen amplicons were observed in 8 tumours of all grades, of which the most common were localised to 7q31 (4 cases), 8q21-22 (3 cases), 19p (2 cases), 2q (2 cases), and 12q15-21 (2 cases). From this study, it appears that paediatric glial tumours are much more genetically heterogeneous than their adult counterparts, and further molecular investigations are needed to define clinically useful subgroups.
Citation:
Neuro-oncology, 5(1): 69
Publisher:
Society for Neuro-Oncology and Duke University Press
Journal:
Neuro-oncology
Issue Date:
2003
URI:
http://hdl.handle.net/2436/29985
Additional Links:
http://neuro-oncology.dukejournals.org/
Type:
Meetings & Proceedings
Language:
en
Description:
Abstracts from Neuro-Oncology are provided here courtesy of Society for Neuro-Oncology and Duke University Press.
ISSN:
1522-8517
Appears in Collections:
Cancer Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorWard, Samantha-
dc.contributor.authorHayward, Richard-
dc.contributor.authorHarkness, William-
dc.contributor.authorPhipps, Kim-
dc.contributor.authorThompson, Dominic-
dc.contributor.authorHarding, Brian-
dc.contributor.authorWilkins, Peter-
dc.contributor.authorDarling, John L.-
dc.contributor.authorThomas, David G.-
dc.contributor.authorWarr, Tracy-
dc.date.accessioned2008-06-12T14:45:36Z-
dc.date.available2008-06-12T14:45:36Z-
dc.date.issued2003-
dc.identifier.citationNeuro-oncology, 5(1): 69en
dc.identifier.issn1522-8517-
dc.identifier.urihttp://hdl.handle.net/2436/29985-
dc.descriptionAbstracts from Neuro-Oncology are provided here courtesy of Society for Neuro-Oncology and Duke University Press.en
dc.description.abstractGlial cell tumours represent the largest group of brain tumours in childhood and include astrocytoma (WHO grades I-IV) and ependymoma (WHO grade II-III). However, little is known about the pathogenesis of these tumours. We have used comparative genomic hybridisation (CGH) to investigate the genetic alterations in 128 tumours from children and young adults (< 30 years of age) comprising 52 ependymoma, including 40 samples that have previously been reported (44 grade II and 8 grade III) and 76 astrocytoma (consisting of 34 grade I, 17 grade II, 7 grade III, and 18 grade IV). Genetic alterations were compared to clinicopathological data such as histology, tumour recurrence, and survival in order to identify potential prognostic markers. In ependymoma, 39% of the tumours had no detectable copy number aberrations (CNAs). In the remaining tumours, the most common regions of gain were 4q (29%), 6q (21%), 1q (17%), and 2q (15%). The most common regions of loss were 22 (29%), 16p (17%), 17p (13%), and 20q (13%). Three regions of high copy number amplification were observed in 3 tumours at 1q24-31 (3 cases), 8q21-23 (3 cases), and 9p (1 case). There was no association between any CNA and histology, tumour recurrence, or length of survival. In contrast, in the astrocytoma group there was a clear association between histology and the presence of CNAs. The pattern of genetic alterations became increasingly complex with tumour grade, and grade IV tumours were more likely to have CNAs than lower grade tumours (p = 0.0502). Overall, the most frequent alterations observed in astrocytoma were gain of 4q (11%), loss 16p (10.5%), and loss 17p (10.5%). However, several CNAs were seen predominantly in grade IV tumours (gain 1q, 2q, 4q, and 5q). Fourteen amplicons were observed in 8 tumours of all grades, of which the most common were localised to 7q31 (4 cases), 8q21-22 (3 cases), 19p (2 cases), 2q (2 cases), and 12q15-21 (2 cases). From this study, it appears that paediatric glial tumours are much more genetically heterogeneous than their adult counterparts, and further molecular investigations are needed to define clinically useful subgroups.en
dc.language.isoenen
dc.publisherSociety for Neuro-Oncology and Duke University Pressen
dc.relation.urlhttp://neuro-oncology.dukejournals.org/en
dc.subjectGliomaen
dc.subjectCGHen
dc.subjectComparative Genomic Hybridisationen
dc.subjectBrain Tumoursen
dc.subjectMalignant tumoursen
dc.subjectOncologyen
dc.subjectChildrenen
dc.subjectPaediatric tumoursen
dc.subjectCancer geneticsen
dc.subjectGenomicsen
dc.subjectMolecular Biologyen
dc.subjectTumor Cells, Cultureden
dc.subjectGene Expressionen
dc.subjectChromosomal Aberrationsen
dc.subjectCell Cultureen
dc.subjectCancer progressionen
dc.titleCorrelation of copy number aberrations with clinico-pathological criteria in paediatric glial tumoursen
dc.title.alternativeIn: Tenth International Symposium on Pediatric Neuro-Oncology, June 9–12, 2002, London, England. No.194en
dc.typeMeetings & Proceedingsen
dc.identifier.journalNeuro-oncologyen
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