Development and application of RT-PCR systems to determine HERV expression in astrocytoma cell lines

2.50
Hdl Handle:
http://hdl.handle.net/2436/29972
Title:
Development and application of RT-PCR systems to determine HERV expression in astrocytoma cell lines
Authors:
Nelson, Paul N.; Smith, R.; Conde, Gillian; Roden, Denise A.; Darling, John L.
Other Titles:
In: Abstracts from the World Federation of Neuro-Oncology Second Quadrennial Meeting and the Sixth Meeting of the European Association for Neuro-Oncology: May 5–8, 2005, Edinburgh, UK. No.174
Abstract:
Human endogenous retroviruses (HERVs) belong to the family of transposable elements that make up 8% of the human genome. Unlike exogenous retrovirus (e.g., HIV and HTLV), HERVs are inherited in a Mendelian manner. More than 22 families of HERVs have been identified over the past two decades. Importantly, some HERVs have been found to possess large open reading frames and produce viral like particles. More latterly, these viruses have been linked with certain autoimmune diseases and cancers. Indeed, HERVs may contribute toward carcinogenesis through retrotransposition, promoter insertion, immunomodulation, disruption of normal HERV-related functions, recombination, or by the production of fusion proteins. Of importance, HERV-K, HERV-W, and HERV-H have the potential to be transcriptionally active in the brain. We have developed robust RT-PCR systems using primers/probes specific to HERV-K and HERV-W to assess mRNA expression in conjunction with the house keeping gene, histidyl tRNA synthetase. In employing a gel-documentation system, we are able to provide semiquantitative levels of HERV expression in cell lines. Pilot data shows markedly enhanced expression of HERV-K in the cell line U251-MG (derived from a glioblastoma multiforme; WHO grade IV astrocytoma) as compared to a control cell line SW480 (colon adenocarcinoma): RT-PCR values; 1.0 and 0.42, respectively. This observation raises an intriguing possibility that HERV-K expression may be elevated in malignant brain tumors. In addition, this approach provides a useful approach to optimize primers and probes prior to using real-time quantitative PCR.
Citation:
Neuro-oncology, 7: 325
Publisher:
Society for Neuro-Oncology and Duke University Press
Journal:
Neuro-oncology
Issue Date:
2005
URI:
http://hdl.handle.net/2436/29972
Additional Links:
http://neuro-oncology.dukejournals.org/; http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1871910
Type:
Meetings & Proceedings
Language:
en
Description:
Abstracts from Neuro-Oncology are provided here courtesy of Society for Neuro-Oncology and Duke University Press.
ISSN:
1522-8517
Appears in Collections:
Cancer Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorNelson, Paul N.-
dc.contributor.authorSmith, R.-
dc.contributor.authorConde, Gillian-
dc.contributor.authorRoden, Denise A.-
dc.contributor.authorDarling, John L.-
dc.date.accessioned2008-06-12T12:27:41Z-
dc.date.available2008-06-12T12:27:41Z-
dc.date.issued2005-
dc.identifier.citationNeuro-oncology, 7: 325en
dc.identifier.issn1522-8517-
dc.identifier.urihttp://hdl.handle.net/2436/29972-
dc.descriptionAbstracts from Neuro-Oncology are provided here courtesy of Society for Neuro-Oncology and Duke University Press.en
dc.description.abstractHuman endogenous retroviruses (HERVs) belong to the family of transposable elements that make up 8% of the human genome. Unlike exogenous retrovirus (e.g., HIV and HTLV), HERVs are inherited in a Mendelian manner. More than 22 families of HERVs have been identified over the past two decades. Importantly, some HERVs have been found to possess large open reading frames and produce viral like particles. More latterly, these viruses have been linked with certain autoimmune diseases and cancers. Indeed, HERVs may contribute toward carcinogenesis through retrotransposition, promoter insertion, immunomodulation, disruption of normal HERV-related functions, recombination, or by the production of fusion proteins. Of importance, HERV-K, HERV-W, and HERV-H have the potential to be transcriptionally active in the brain. We have developed robust RT-PCR systems using primers/probes specific to HERV-K and HERV-W to assess mRNA expression in conjunction with the house keeping gene, histidyl tRNA synthetase. In employing a gel-documentation system, we are able to provide semiquantitative levels of HERV expression in cell lines. Pilot data shows markedly enhanced expression of HERV-K in the cell line U251-MG (derived from a glioblastoma multiforme; WHO grade IV astrocytoma) as compared to a control cell line SW480 (colon adenocarcinoma): RT-PCR values; 1.0 and 0.42, respectively. This observation raises an intriguing possibility that HERV-K expression may be elevated in malignant brain tumors. In addition, this approach provides a useful approach to optimize primers and probes prior to using real-time quantitative PCR.en
dc.language.isoenen
dc.publisherSociety for Neuro-Oncology and Duke University Pressen
dc.relation.urlhttp://neuro-oncology.dukejournals.org/en
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1871910en
dc.subjectBrain Tumoursen
dc.subjectHuman Endogenous Retrovirusesen
dc.subjectGenomicsen
dc.subjectHERVsen
dc.subjectCarcinogenesisen
dc.subjectRNA, Messengeren
dc.subjectCell Line, Tumoren
dc.subjectU251MGen
dc.subjectAstrocytomaen
dc.subjectGlioblastomaen
dc.subjectMalignant tumoursen
dc.subjectPCRen
dc.subjectOncologyen
dc.subjectChemotherapeutic targetsen
dc.subjectCancer geneticsen
dc.subjectRNA, Viralen
dc.titleDevelopment and application of RT-PCR systems to determine HERV expression in astrocytoma cell linesen
dc.title.alternativeIn: Abstracts from the World Federation of Neuro-Oncology Second Quadrennial Meeting and the Sixth Meeting of the European Association for Neuro-Oncology: May 5–8, 2005, Edinburgh, UK. No.174en
dc.typeMeetings & Proceedingsen
dc.identifier.journalNeuro-oncologyen
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