Reduced expression of endothelial and inducible nitric oxide synthase in a multidrug resistant variant of the MCF-7 human breast cancer cell line.

2.50
Hdl Handle:
http://hdl.handle.net/2436/29764
Title:
Reduced expression of endothelial and inducible nitric oxide synthase in a multidrug resistant variant of the MCF-7 human breast cancer cell line.
Authors:
Lahiri, M.; Martin, Jan H.
Abstract:
The purpose of the present study was to investigate expression of endothelial and inducible nitric oxide synthase in the MCF-7 human breast cancer cell line compared to a multidrug resistant variant, MCF-7-ADR. Immunohistochemical investigations demonstrated 51% of MCF-7 cells stained positive for endothelial nitric oxide synthase, with 46% positive for inducible nitric oxide synthase. However, in the breast cancer cell line that was multidrug resistant there was a much lower positive staining for endothelial nitric oxide synthase at 20% and for inducible nitric oxide synthase at 15%. For the multidrug resistant variant, there was also lower nitric oxide production and the band intensity for immunoblotting for endothelial nitric oxide synthase was weaker than for the parent cell line. These results lend further support to the proposal that expression of NOS is negatively associated with human breast cancer progression.
Citation:
Oncology Reports, 12 (5): 1007-1011
Publisher:
Spandidos Publications Ltd
Journal:
Oncology Reports
Issue Date:
2004
URI:
http://hdl.handle.net/2436/29764
PubMed ID:
15492785
Additional Links:
http://direct.bl.uk/bld/PlaceOrder.do?UIN=157575889&ETOC=RN&from=searchengine
Type:
Article
Language:
en
ISSN:
1021-335X
Appears in Collections:
Cancer Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLahiri, M.-
dc.contributor.authorMartin, Jan H.-
dc.date.accessioned2008-06-10T10:48:06Z-
dc.date.available2008-06-10T10:48:06Z-
dc.date.issued2004-
dc.identifier.citationOncology Reports, 12 (5): 1007-1011en
dc.identifier.issn1021-335X-
dc.identifier.pmid15492785-
dc.identifier.urihttp://hdl.handle.net/2436/29764-
dc.description.abstractThe purpose of the present study was to investigate expression of endothelial and inducible nitric oxide synthase in the MCF-7 human breast cancer cell line compared to a multidrug resistant variant, MCF-7-ADR. Immunohistochemical investigations demonstrated 51% of MCF-7 cells stained positive for endothelial nitric oxide synthase, with 46% positive for inducible nitric oxide synthase. However, in the breast cancer cell line that was multidrug resistant there was a much lower positive staining for endothelial nitric oxide synthase at 20% and for inducible nitric oxide synthase at 15%. For the multidrug resistant variant, there was also lower nitric oxide production and the band intensity for immunoblotting for endothelial nitric oxide synthase was weaker than for the parent cell line. These results lend further support to the proposal that expression of NOS is negatively associated with human breast cancer progression.en
dc.language.isoenen
dc.publisherSpandidos Publications Ltden
dc.relation.urlhttp://direct.bl.uk/bld/PlaceOrder.do?UIN=157575889&ETOC=RN&from=searchengine-
dc.subject.meshAntibiotics, Antineoplasticen
dc.subject.meshBreast Neoplasmsen
dc.subject.meshDoxorubicinen
dc.subject.meshDrug Resistance, Multipleen
dc.subject.meshDrug Resistance, Neoplasmen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshNitric Oxideen
dc.subject.meshNitric Oxide Synthaseen
dc.subject.meshNitric Oxide Synthase Type IIen
dc.subject.meshNitric Oxide Synthase Type IIIen
dc.subject.meshTumor Cells, Cultureden
dc.titleReduced expression of endothelial and inducible nitric oxide synthase in a multidrug resistant variant of the MCF-7 human breast cancer cell line.en
dc.typeArticleen
dc.identifier.journalOncology Reportsen
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