Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.

2.50
Hdl Handle:
http://hdl.handle.net/2436/29757
Title:
Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.
Authors:
Yi, Tao; Leung, Kelvin Sze-Yin; Lu, Guang-Hua; Chan, Kelvin C.; Zhang, Hao
Abstract:
An HPLC-DAD-MS method was developed for the qualitative and quantitative analysis of the major constituents in Chuanxiong (the dried rhizome of Ligusticum chuanxiong Hort). Twenty compounds including phenolic constituents, alkylphthalides and phthalide dimers were identified using online ESI-MS and comparisons with literature data and standard compounds, and six of them were quantified by HPLC-DAD simultaneously. A comprehensive validation of the method including sensitivity, linearity, repeatability and recovery was conducted. The linear regressions were acquired with R(2) > 0.99 and limit of detection (LOD, S/N = 3) values were between 1.5 and 2.5 ng. The repeatability was evaluated by intra- and inter-day assays, and relative standard deviation (RSD) values were reported within 1.87%. The recovery studies for the quantified compounds were observed in the range of 96.36-102.37% with RSD values less than 2.63%. These phenolic constituents and alkylphthalides, the major constituents in Chuanxiong, are generally regarded as the index for the quality assessment of this herb. The overall procedure is accurate and reproducible, which is considered suitable for the qualitative and quantitative analyses of a large number of Chuanxiong samples.
Citation:
Chemical & Pharmaceutical Bulletin, 54(2): 255-259
Publisher:
Tokyo: The Pharmaceutical Society of Japan
Journal:
Chemical & Pharmaceutical Bulletin
Issue Date:
2006
URI:
http://hdl.handle.net/2436/29757
PubMed ID:
16462078
Additional Links:
http://www.jstage.jst.go.jp/article/cpb/54/2/54_255/_article
Type:
Article
Language:
en
ISSN:
0009-2363; 1347-5223
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorYi, Tao-
dc.contributor.authorLeung, Kelvin Sze-Yin-
dc.contributor.authorLu, Guang-Hua-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorZhang, Hao-
dc.date.accessioned2008-06-10T09:41:55Z-
dc.date.available2008-06-10T09:41:55Z-
dc.date.issued2006-
dc.identifier.citationChemical & Pharmaceutical Bulletin, 54(2): 255-259en
dc.identifier.issn0009-2363-
dc.identifier.issn1347-5223-
dc.identifier.pmid16462078-
dc.identifier.urihttp://hdl.handle.net/2436/29757-
dc.description.abstractAn HPLC-DAD-MS method was developed for the qualitative and quantitative analysis of the major constituents in Chuanxiong (the dried rhizome of Ligusticum chuanxiong Hort). Twenty compounds including phenolic constituents, alkylphthalides and phthalide dimers were identified using online ESI-MS and comparisons with literature data and standard compounds, and six of them were quantified by HPLC-DAD simultaneously. A comprehensive validation of the method including sensitivity, linearity, repeatability and recovery was conducted. The linear regressions were acquired with R(2) > 0.99 and limit of detection (LOD, S/N = 3) values were between 1.5 and 2.5 ng. The repeatability was evaluated by intra- and inter-day assays, and relative standard deviation (RSD) values were reported within 1.87%. The recovery studies for the quantified compounds were observed in the range of 96.36-102.37% with RSD values less than 2.63%. These phenolic constituents and alkylphthalides, the major constituents in Chuanxiong, are generally regarded as the index for the quality assessment of this herb. The overall procedure is accurate and reproducible, which is considered suitable for the qualitative and quantitative analyses of a large number of Chuanxiong samples.en
dc.language.isoenen
dc.publisherTokyo: The Pharmaceutical Society of Japanen
dc.relation.urlhttp://www.jstage.jst.go.jp/article/cpb/54/2/54_255/_articleen
dc.subjectLigusticum Chuanxiong Horten
dc.subjectPhenolic constituenten
dc.subjectAlkylphthalideen
dc.subjectHPLC-DAD-MSen
dc.subjectChuanxiong Rhizomeen
dc.subjectHerbal medicineen
dc.subjectChinese medicineen
dc.subjectTraditional Chinese medicineen
dc.subjectMedicine, Chinese Traditional-
dc.subject.meshCalibrationen
dc.subject.meshChromatography, High Pressure Liquiden
dc.subject.meshIndicators and Reagentsen
dc.subject.meshLigusticumen
dc.subject.meshMass Spectrometryen
dc.subject.meshPlant Rootsen
dc.subject.meshReference Standardsen
dc.subject.meshReproducibility of Resultsen
dc.subject.meshSpectrophotometry, Ultravioleten
dc.titleSimultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.en
dc.typeArticleen
dc.identifier.journalChemical & Pharmaceutical Bulletinen
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