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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Pharmacy and Natural Products Research Group > Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/29757
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Title: Simultaneous qualitative and quantitative analyses of the major constituents in the rhizome of Ligusticum Chuanxiong using HPLC-DAD-MS.
Authors: Yi, Tao
Leung, Kelvin Sze-Yin
Lu, Guang-Hua
Chan, Kelvin C.
Zhang, Hao
Citation: Chemical & Pharmaceutical Bulletin, 54(2): 255-259
Publisher: Tokyo: The Pharmaceutical Society of Japan
Journal: Chemical & Pharmaceutical Bulletin
Issue Date: 2006
URI: http://hdl.handle.net/2436/29757
PubMed ID: 16462078
Additional Links: http://www.jstage.jst.go.jp/article/cpb/54/2/54_255/_article
Abstract: An HPLC-DAD-MS method was developed for the qualitative and quantitative analysis of the major constituents in Chuanxiong (the dried rhizome of Ligusticum chuanxiong Hort). Twenty compounds including phenolic constituents, alkylphthalides and phthalide dimers were identified using online ESI-MS and comparisons with literature data and standard compounds, and six of them were quantified by HPLC-DAD simultaneously. A comprehensive validation of the method including sensitivity, linearity, repeatability and recovery was conducted. The linear regressions were acquired with R(2) > 0.99 and limit of detection (LOD, S/N = 3) values were between 1.5 and 2.5 ng. The repeatability was evaluated by intra- and inter-day assays, and relative standard deviation (RSD) values were reported within 1.87%. The recovery studies for the quantified compounds were observed in the range of 96.36-102.37% with RSD values less than 2.63%. These phenolic constituents and alkylphthalides, the major constituents in Chuanxiong, are generally regarded as the index for the quality assessment of this herb. The overall procedure is accurate and reproducible, which is considered suitable for the qualitative and quantitative analyses of a large number of Chuanxiong samples.
Type: Article
Language: en
Keywords: Ligusticum Chuanxiong Hort
Phenolic constituent
Alkylphthalide
HPLC-DAD-MS
Chuanxiong Rhizome
Herbal medicine
Chinese medicine
Traditional Chinese medicine
Medicine, Chinese Traditional
MeSH: Calibration
Chromatography, High Pressure Liquid
Indicators and Reagents
Ligusticum
Mass Spectrometry
Plant Roots
Reference Standards
Reproducibility of Results
Spectrophotometry, Ultraviolet
ISSN: 0009-2363
1347-5223
Appears in Collections: Pharmacy and Natural Products Research Group

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