High Performance liquid chromatography-mass spectrometry analysis for rat metabolism and pharmacokinetic studies of lithospermic acid B from danshen

2.50
Hdl Handle:
http://hdl.handle.net/2436/29754
Title:
High Performance liquid chromatography-mass spectrometry analysis for rat metabolism and pharmacokinetic studies of lithospermic acid B from danshen
Authors:
Cui, Liang; Chan, Wan; Wu, Jian-Lin; Jiang, Zhi-Hong; Chan, Kelvin C.; Cai, Zongwei
Abstract:
Metabolism and pharmacokinetic studies on rat were conducted for lithospermic acid B, one of the components from Radix Salviae Miltiorrhizae (danshen) that shows many bioactivities. Liquid chromatography–electrospray ionization mass spectrometry method was applied for the determination of lithospermic acid B and its metabolites in samples from in vitro and in vivo metabolism studies. Rat plasma samples collected after intravenous administration were analyzed for obtaining pharmacokinetic data of lithospermic acid B. Four O-methylated metabolites, namely one monomethyl-, two dimethyl- and one trimethyl-lithospermic acid B, were detected when lithospermic acid B was incubated in rat hepatic cytosol. These four metabolites were also detected in rat bile, plasma and feces samples after intravenous administration of lithospermic acid B. The in vitro and in vivo results indicate that the methylation is the main metabolic pathway of lithospermic acid B. The danshen component and its methylated metabolites were excreted to rat bile and feces. (Elsevier)
Citation:
Talanta, 75(4): 1002-1007
Publisher:
Amsterdam: Elsevier
Journal:
Talanta
Issue Date:
2008
URI:
http://hdl.handle.net/2436/29754
DOI:
10.1016/j.talanta.2007.12.045
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4RKMHW4-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ae9ff64b23dc55ac9e68d5a60cb0bb1d
Type:
Article
Language:
en
ISSN:
00399140
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorCui, Liang-
dc.contributor.authorChan, Wan-
dc.contributor.authorWu, Jian-Lin-
dc.contributor.authorJiang, Zhi-Hong-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorCai, Zongwei-
dc.date.accessioned2008-06-10T09:31:43Z-
dc.date.available2008-06-10T09:31:43Z-
dc.date.issued2008-
dc.identifier.citationTalanta, 75(4): 1002-1007en
dc.identifier.issn00399140-
dc.identifier.doi10.1016/j.talanta.2007.12.045-
dc.identifier.urihttp://hdl.handle.net/2436/29754-
dc.description.abstractMetabolism and pharmacokinetic studies on rat were conducted for lithospermic acid B, one of the components from Radix Salviae Miltiorrhizae (danshen) that shows many bioactivities. Liquid chromatography–electrospray ionization mass spectrometry method was applied for the determination of lithospermic acid B and its metabolites in samples from in vitro and in vivo metabolism studies. Rat plasma samples collected after intravenous administration were analyzed for obtaining pharmacokinetic data of lithospermic acid B. Four O-methylated metabolites, namely one monomethyl-, two dimethyl- and one trimethyl-lithospermic acid B, were detected when lithospermic acid B was incubated in rat hepatic cytosol. These four metabolites were also detected in rat bile, plasma and feces samples after intravenous administration of lithospermic acid B. The in vitro and in vivo results indicate that the methylation is the main metabolic pathway of lithospermic acid B. The danshen component and its methylated metabolites were excreted to rat bile and feces. (Elsevier)en
dc.language.isoenen
dc.publisherAmsterdam: Elsevieren
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4RKMHW4-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ae9ff64b23dc55ac9e68d5a60cb0bb1den
dc.subjectLithospermic acid Ben
dc.subjectDrug metabolismen
dc.subjectPharmacokineticsen
dc.subjectRatsen
dc.subjectDanshenen
dc.subjectChromatography, High Performance Liquiden
dc.subjectHaematology, animalen
dc.subjectHPLCen
dc.titleHigh Performance liquid chromatography-mass spectrometry analysis for rat metabolism and pharmacokinetic studies of lithospermic acid B from danshenen
dc.typeArticleen
dc.identifier.journalTalantaen
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