Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.

2.50
Hdl Handle:
http://hdl.handle.net/2436/29678
Title:
Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.
Authors:
Yu, Hua; Ho, Jason; Kan, Kelvin K.; Cheng, Bobby; Li, Wen-Ming; Zhang, Li; Lin, Ge; Pang, Yuan-Ping; Gu, Zhe-Ming; Chan, Kelvin C.; Wang, Yi-Tao; Han, Yi-Fan
Abstract:
An analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100 microl of alkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25 degrees C. The elution was achieved isocratically with a mobile phase composed of 0.05% aqueous formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.35 ml/min. Quantification was achieved by monitoring the selected ions at m/z 247 for B7T and m/z 462-->m/z 328 for pimozide. Retention times were 1.45 and 2.23 min for B7T and IS, respectively. Calibration curves were linear in the range from 86.4 to 2160.0 ng/ml. The established method is rapid, selective and sensitive for the identification and quantification of B7T in biological samples. The assay is accurate (bias <10%) and reproducible (intra- and inter-day variation <10%), with detection and quantification limit of 3.6 and 42.3 ng/ml, respectively. Furthermore, it was successfully applied for the pharmacokinetic measurement of B7T in rat with a single intravenous administration at 0.3mg/kg.
Citation:
Journal of pharmaceutical and biomedical analysis, 44(5): 1133-1188
Publisher:
Amsterdam: Elsevier
Journal:
Journal of pharmaceutical and biomedical analysis
Issue Date:
2007
URI:
http://hdl.handle.net/2436/29678
DOI:
10.1016/j.jpba.2007.05.028
PubMed ID:
17628383
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4NTJH0F-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ea1d89987a34d1285fa1f7f826ca1307
Type:
Article
Language:
en
ISSN:
0731-7085
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorYu, Hua-
dc.contributor.authorHo, Jason-
dc.contributor.authorKan, Kelvin K.-
dc.contributor.authorCheng, Bobby-
dc.contributor.authorLi, Wen-Ming-
dc.contributor.authorZhang, Li-
dc.contributor.authorLin, Ge-
dc.contributor.authorPang, Yuan-Ping-
dc.contributor.authorGu, Zhe-Ming-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorWang, Yi-Tao-
dc.contributor.authorHan, Yi-Fan-
dc.date.accessioned2008-06-09T16:18:55Z-
dc.date.available2008-06-09T16:18:55Z-
dc.date.issued2007-
dc.identifier.citationJournal of pharmaceutical and biomedical analysis, 44(5): 1133-1188en
dc.identifier.issn0731-7085-
dc.identifier.pmid17628383-
dc.identifier.doi10.1016/j.jpba.2007.05.028-
dc.identifier.urihttp://hdl.handle.net/2436/29678-
dc.description.abstractAn analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100 microl of alkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25 degrees C. The elution was achieved isocratically with a mobile phase composed of 0.05% aqueous formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.35 ml/min. Quantification was achieved by monitoring the selected ions at m/z 247 for B7T and m/z 462-->m/z 328 for pimozide. Retention times were 1.45 and 2.23 min for B7T and IS, respectively. Calibration curves were linear in the range from 86.4 to 2160.0 ng/ml. The established method is rapid, selective and sensitive for the identification and quantification of B7T in biological samples. The assay is accurate (bias <10%) and reproducible (intra- and inter-day variation <10%), with detection and quantification limit of 3.6 and 42.3 ng/ml, respectively. Furthermore, it was successfully applied for the pharmacokinetic measurement of B7T in rat with a single intravenous administration at 0.3mg/kg.en
dc.language.isoenen
dc.publisherAmsterdam: Elsevieren
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4NTJH0F-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ea1d89987a34d1285fa1f7f826ca1307en
dc.subjectPharmacokineticsen
dc.subjectNeuropharmacologyen
dc.subject.meshAlzheimer Diseaseen
dc.subject.meshAnimalsen
dc.subject.meshArea Under Curveen
dc.subject.meshChromatography, High Pressure Liquiden
dc.subject.meshDrug Stabilityen
dc.subject.meshFreezingen
dc.subject.meshHalf-Lifeen
dc.subject.meshInjections, Intravenousen
dc.subject.meshMaleen
dc.subject.meshMetabolic Clearance Rateen
dc.subject.meshMolecular Structureen
dc.subject.meshRatsen
dc.subject.meshRats, Sprague-Dawleyen
dc.subject.meshReproducibility of Resultsen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshTacrineen
dc.subject.meshTandem Mass Spectrometryen
dc.subject.meshTime Factorsen
dc.titleDevelopment of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.en
dc.typeArticleen
dc.identifier.journalJournal of pharmaceutical and biomedical analysisen
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