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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Pharmacy and Natural Products Research Group > Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/29678
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Title: Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.
Authors: Yu, Hua
Ho, Jason
Kan, Kelvin K.
Cheng, Bobby
Li, Wen-Ming
Zhang, Li
Lin, Ge
Pang, Yuan-Ping
Gu, Zhe-Ming
Chan, Kelvin C.
Wang, Yi-Tao
Han, Yi-Fan
Citation: Journal of pharmaceutical and biomedical analysis, 44(5): 1133-1188
Publisher: Amsterdam: Elsevier
Journal: Journal of pharmaceutical and biomedical analysis
Issue Date: 2007
URI: http://hdl.handle.net/2436/29678
DOI: 10.1016/j.jpba.2007.05.028
PubMed ID: 17628383
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4NTJH0F-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ea1d89987a34d1285fa1f7f826ca1307
Abstract: An analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100 microl of alkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25 degrees C. The elution was achieved isocratically with a mobile phase composed of 0.05% aqueous formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.35 ml/min. Quantification was achieved by monitoring the selected ions at m/z 247 for B7T and m/z 462-->m/z 328 for pimozide. Retention times were 1.45 and 2.23 min for B7T and IS, respectively. Calibration curves were linear in the range from 86.4 to 2160.0 ng/ml. The established method is rapid, selective and sensitive for the identification and quantification of B7T in biological samples. The assay is accurate (bias <10%) and reproducible (intra- and inter-day variation <10%), with detection and quantification limit of 3.6 and 42.3 ng/ml, respectively. Furthermore, it was successfully applied for the pharmacokinetic measurement of B7T in rat with a single intravenous administration at 0.3mg/kg.
Type: Article
Language: en
Keywords: Pharmacokinetics
Neuropharmacology
MeSH: Alzheimer Disease
Animals
Area Under Curve
Chromatography, High Pressure Liquid
Drug Stability
Freezing
Half-Life
Injections, Intravenous
Male
Metabolic Clearance Rate
Molecular Structure
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Tacrine
Tandem Mass Spectrometry
Time Factors
ISSN: 0731-7085
Appears in Collections: Pharmacy and Natural Products Research Group

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