2.50
Hdl Handle:
http://hdl.handle.net/2436/29473
Title:
Increased non-transferrin bound iron in plasma-depleted SAG-M red blood cell units.
Authors:
Marwah, S.S.; Blann, A.D.; Harrison, P.; Lumley, M.A.; Wright, J.; McDowell, J.; Phillips, Jonathan D.; Rea, C.; Bareford, D.
Abstract:
BACKGROUND AND OBJECTIVES: Non-transferrin bound iron (NTBI) is associated with increased morbidity in a number of transfusion-dependent disease states such as the severe haemoglobinopathies. We hypothesized that this may be related to excess NTBI present in plasma-depleted red blood cell units that are free of clear haemolysis. MATERIALS AND METHODS: The level of NTBI was determined using the bleomycin assay in samples from 20 stored plasma-depleted red cell units, at approximate 5-day intervals up to day 33 after donation. Forty units of fresh-frozen plasma (FFP) and 40 units of platelet concentrates were used as negative controls, and samples from 12 units of FFP were also serially assessed. RESULTS: Median [interquartile range (IQR)] NTBI was 0 microm (0-0.35) in samples taken from units 3-10 days after donation. Thereafter, the levels of NTBI increased, becoming significant (median 3.05; IQR: 0.05-6.7 microm) 17-22 days after donation. After 30 days, NTBI was detectable in all red cell units. NTBI was undetectable in platelet concentrates and FFP. CONCLUSIONS: Increased levels of NTBI become detectable 17-22 days after donation and increase further with storage time. This excess NTBI may promote bacterial infection in iron-loaded individuals.
Citation:
Vox Sanguinis, 82(3): 122-126
Publisher:
Wiley InterScience
Journal:
Vox Sanguinis
Issue Date:
2002
URI:
http://hdl.handle.net/2436/29473
DOI:
10.1046/j.1423-0410.2002.00153.x
PubMed ID:
11952985
Additional Links:
http://www3.interscience.wiley.com/journal/118912470/abstract; http://www.ingentaconnect.com/content/bsc/vox/2002/00000082/00000003/art00003; http://direct.bl.uk/bld/PlaceOrder.do?UIN=110927228&ETOC=RN&from=searchengine
Type:
Article
Language:
en
ISSN:
0042-9007
Appears in Collections:
Diabetes, Physiology and Molecular Medicine Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorMarwah, S.S.-
dc.contributor.authorBlann, A.D.-
dc.contributor.authorHarrison, P.-
dc.contributor.authorLumley, M.A.-
dc.contributor.authorWright, J.-
dc.contributor.authorMcDowell, J.-
dc.contributor.authorPhillips, Jonathan D.-
dc.contributor.authorRea, C.-
dc.contributor.authorBareford, D.-
dc.date.accessioned2008-06-04T11:17:52Z-
dc.date.available2008-06-04T11:17:52Z-
dc.date.issued2002-
dc.identifier.citationVox Sanguinis, 82(3): 122-126en
dc.identifier.issn0042-9007-
dc.identifier.pmid11952985-
dc.identifier.doi10.1046/j.1423-0410.2002.00153.x-
dc.identifier.urihttp://hdl.handle.net/2436/29473-
dc.description.abstractBACKGROUND AND OBJECTIVES: Non-transferrin bound iron (NTBI) is associated with increased morbidity in a number of transfusion-dependent disease states such as the severe haemoglobinopathies. We hypothesized that this may be related to excess NTBI present in plasma-depleted red blood cell units that are free of clear haemolysis. MATERIALS AND METHODS: The level of NTBI was determined using the bleomycin assay in samples from 20 stored plasma-depleted red cell units, at approximate 5-day intervals up to day 33 after donation. Forty units of fresh-frozen plasma (FFP) and 40 units of platelet concentrates were used as negative controls, and samples from 12 units of FFP were also serially assessed. RESULTS: Median [interquartile range (IQR)] NTBI was 0 microm (0-0.35) in samples taken from units 3-10 days after donation. Thereafter, the levels of NTBI increased, becoming significant (median 3.05; IQR: 0.05-6.7 microm) 17-22 days after donation. After 30 days, NTBI was detectable in all red cell units. NTBI was undetectable in platelet concentrates and FFP. CONCLUSIONS: Increased levels of NTBI become detectable 17-22 days after donation and increase further with storage time. This excess NTBI may promote bacterial infection in iron-loaded individuals.en
dc.language.isoenen
dc.publisherWiley InterScienceen
dc.relation.urlhttp://www3.interscience.wiley.com/journal/118912470/abstracten
dc.relation.urlhttp://www.ingentaconnect.com/content/bsc/vox/2002/00000082/00000003/art00003-
dc.relation.urlhttp://direct.bl.uk/bld/PlaceOrder.do?UIN=110927228&ETOC=RN&from=searchengine-
dc.subjectBacterial contaminationen
dc.subjectFresh-frozen plasmaen
dc.subjectBlood Transfusionen
dc.subjectNontransferrin bound iron (NTBI)en
dc.subjectPlateletsen
dc.subjectSAG-Men
dc.subject.meshBacterial Infectionsen
dc.subject.meshBlood Preservationen
dc.subject.meshErythrocyte Transfusionen
dc.subject.meshErythrocytesen
dc.subject.meshHumansen
dc.subject.meshIron Overloaden
dc.subject.meshPlasma Substitutesen
dc.subject.meshTime Factorsen
dc.subject.meshTransferrinen
dc.titleIncreased non-transferrin bound iron in plasma-depleted SAG-M red blood cell units.en
dc.typeArticleen
dc.identifier.journalVox Sanguinisen

Related articles on PubMed

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.