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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Cancer Research Group > Interactions of cell penetrating peptide Tat with model membranes: a biophysical study.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/29463
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Title: Interactions of cell penetrating peptide Tat with model membranes: a biophysical study.
Authors: Dennison, Sarah R.
Baker, Rachael D.
Nicholl, Iain D.
Phoenix, David A.
Citation: Biochemical and Biophysical Research Communications, 363(1): 178-182
Publisher: Elsevier Science Direct
Journal: Biochemical and Biophysical Research Communications
Issue Date: 2007
URI: http://hdl.handle.net/2436/29463
DOI: 10.1016/j.bbrc.2007.08.162
PubMed ID: 17854767
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PK8K5N-7&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=79df9af6f737d67fceb156556b328c01
Abstract: The protein transduction domain of the HIV-1 transactivator of transcription, Tat (Tat((48-60))), has been shown to transport P10, a cytotoxic peptide mimic of the cyclin dependent kinase inhibitor p21WAF1/CIP1, into the nucleus of cancerous cells and induce apoptosis. Here, monolayer studies were used to investigate the membrane interactions of Tat((48-60)), P10 and the construct Tat((48-60))P10. It was found that Tat((48-60)) showed no significant surface activity but that both P10 and Tat((48-60))P10, were highly surface active, inducing surface pressure changes of 9.7 and 8.9mNm(-1), respectively, with DMPS monolayers. The comparison of Tat((48-60))P10 and P10 surface interactions would be consistent with a hypothesis that the cargo attachment influences the capacity of the Tat-protein transduction domain to mediate transport across membranes either directly or via localisation of the construct at the membrane interface.
Type: Article
Language: en
Keywords: Cell Penetrating Peptides (CPP)
Lipid monolayer
Isotherm
Tat peptide
MeSH: Binding Sites
Biomimetics
Biophysics
Lipid Bilayers
Membrane Fluidity
Membranes, Artificial
Peptide Fragments
Phospholipids
Protein Binding
tat Gene Products, Human Immunodeficiency Virus
ISSN: 0006-291X
Appears in Collections: Cancer Research Group

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