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The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.
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|Title: ||The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.|
|Citation: ||Annals of the Rheumatic Diseases, 65 (5): 612-616|
|Publisher: ||BMJ Publishing|
|Journal: ||Annals of the Rheumatic Diseases|
|Issue Date: ||2006 |
|PubMed ID: ||16192292|
|Additional Links: ||http://ard.bmj.com/cgi/content/full/65/5/612|
|Abstract: ||OBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.|
|Keywords: ||Human Endogenous Retroviruses|
Cells, Peripheral Blood Mononuclear
Histidyl tRNA synthetase
Aged, 80 and over
Gene Products, gag
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
|Appears in Collections: ||Molecular Immunology Research Group |
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