The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.

2.50
Hdl Handle:
http://hdl.handle.net/2436/29461
Title:
The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.
Authors:
Ejtehadi, H. Davari; Freimanis, Graham L.; Ali, H.A.; Bowman, S.J.; Alavi, A.; Axford, J.; Callaghan, R.; Nelson, Paul N.
Abstract:
OBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.
Citation:
Annals of the Rheumatic Diseases, 65 (5): 612-616
Publisher:
BMJ Publishing
Journal:
Annals of the Rheumatic Diseases
Issue Date:
2006
URI:
http://hdl.handle.net/2436/29461
DOI:
10.1136/ard.2004.031146
PubMed ID:
16192292
Additional Links:
http://ard.bmj.com/cgi/content/full/65/5/612
Type:
Article
Language:
en
ISSN:
0003-4967
Appears in Collections:
Molecular Immunology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorEjtehadi, H. Davari-
dc.contributor.authorFreimanis, Graham L.-
dc.contributor.authorAli, H.A.-
dc.contributor.authorBowman, S.J.-
dc.contributor.authorAlavi, A.-
dc.contributor.authorAxford, J.-
dc.contributor.authorCallaghan, R.-
dc.contributor.authorNelson, Paul N.-
dc.date.accessioned2008-06-04T10:10:36Z-
dc.date.available2008-06-04T10:10:36Z-
dc.date.issued2006-
dc.identifier.citationAnnals of the Rheumatic Diseases, 65 (5): 612-616en
dc.identifier.issn0003-4967-
dc.identifier.pmid16192292-
dc.identifier.doi10.1136/ard.2004.031146-
dc.identifier.urihttp://hdl.handle.net/2436/29461-
dc.description.abstractOBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.en
dc.language.isoenen
dc.publisherBMJ Publishingen
dc.relation.urlhttp://ard.bmj.com/cgi/content/full/65/5/612en
dc.subjectHuman Endogenous Retrovirusesen
dc.subjectRheumatoid Arthritisen
dc.subjectCells, Peripheral Blood Mononuclearen
dc.subjectHistidyl tRNA synthetaseen
dc.subject.meshAdulten
dc.subject.meshAgeden
dc.subject.meshAged, 80 and overen
dc.subject.meshArthritis, Rheumatoiden
dc.subject.meshAutoimmune Diseasesen
dc.subject.meshBlotting, Southernen
dc.subject.meshDNA, Viralen
dc.subject.meshEndogenous Retrovirusesen
dc.subject.meshGene Expressionen
dc.subject.meshGene Products, gagen
dc.subject.meshHumansen
dc.subject.meshMiddle Ageden
dc.subject.meshOsteoarthritisen
dc.subject.meshRNA, Messengeren
dc.subject.meshRNA, Viralen
dc.subject.meshRetroviridae Infectionsen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshViral Proteinsen
dc.titleThe potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.en
dc.typeArticleen
dc.identifier.journalAnnals of the Rheumatic Diseasesen

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