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Title: The potential role of human endogenous retrovirus K10 in the pathogenesis of rheumatoid arthritis: a preliminary study.
Authors: Ejtehadi, H. Davari
Freimanis, Graham L.
Ali, H.A.
Bowman, S.J.
Alavi, A.
Axford, J.
Callaghan, R.
Nelson, Paul N.
Citation: Annals of the Rheumatic Diseases, 65 (5): 612-616
Publisher: BMJ Publishing
Journal: Annals of the Rheumatic Diseases
Issue Date: 2006
DOI: 10.1136/ard.2004.031146
PubMed ID: 16192292
Additional Links:
Abstract: OBJECTIVE: To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN: A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS: 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals were recruited and total RNA was extracted from peripheral blood mononuclear cells (PBMCs) and analysed using multiplex RT-PCR for the level of HERV-K10 gag mRNA expression. Southern blot and DNA sequencing confirmed the authenticity of the PCR products. RESULTS: Using the histidyl tRNA synthetase (HtRNAS) gene as a housekeeping gene in the optimised multiplex RT-PCR, a significantly higher level of HERV-K10 gag mRNA expression was found in rheumatoid arthritis than in osteoarthritis (p = 0.01) or in the healthy controls (p = 0.02). CONCLUSION: There is enhanced mRNA expression of the HERV-K10 gag region in rheumatoid arthritis compared with osteoarthritis or healthy controls. This could contribute to the pathogenesis of rheumatoid arthritis.
Type: Article
Language: en
Keywords: Human Endogenous Retroviruses
Rheumatoid Arthritis
Cells, Peripheral Blood Mononuclear
Histidyl tRNA synthetase
MeSH: Adult
Aged, 80 and over
Arthritis, Rheumatoid
Autoimmune Diseases
Blotting, Southern
DNA, Viral
Endogenous Retroviruses
Gene Expression
Gene Products, gag
Middle Aged
RNA, Messenger
RNA, Viral
Retroviridae Infections
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Viral Proteins
ISSN: 0003-4967
Appears in Collections: Molecular Immunology Research Group

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