Wolverhampton Intellectual Repository and E-Theses >
Research Institutes >
Research Institute in Healthcare Science >
Food Biology, Medical Microbiology and Disinfection Research Group >
Anti-beta2GPI-antibody-induced endothelial cell gene expression profiling reveals induction of novel pro-inflammatory genes potentially involved in primary antiphospholipid syndrome.
this identifier to cite or link
to this item:
|Title: ||Anti-beta2GPI-antibody-induced endothelial cell gene expression profiling reveals induction of novel pro-inflammatory genes potentially involved in primary antiphospholipid syndrome.|
|Citation: ||Annals of the Rheumatic Diseases, 66 (8): 1000-7|
|Publisher: ||BMJ Publishing & European League Against Rheumatism|
|Journal: ||Annals of the Rheumatic Diseases|
|Issue Date: ||2007 |
|PubMed ID: ||17223652|
|Additional Links: ||http://ard.bmj.com/cgi/content/full/66/8/1000|
|Abstract: ||OBJECTIVE: To determine the effects of primary antiphospholipid syndrome (PAPS)-derived anti-beta(2)GPI antibodies on gene expression in human umbilical vein endothelial cells (HUVEC) by gene profiling using microarrays. METHODS: Anti-beta(2)GPI antibodies purified from sera of patients with PAPS or control IgG isolated from normal subjects were incubated with HUVEC for 4 h before isolation of RNA and processing for hybridisation to Affymetrix Human Genome U133A-2.0 arrays. Data were analysed using a combination of the MAS 5.0 (Affymetrix) and GeneSpring (Agilent) software programmes. For selected genes microarray data were confirmed by real-time PCR analysis or at the protein level by ELISA. RESULTS: A total of 101 genes were found to be upregulated and 14 genes were downregulated twofold or more in response to anti-beta(2)GPI antibodies. A number of novel genes not previously associated with APS were induced, including chemokines CCL20, CXCL3, CX3CL1, CXCL5, CXCL2 and CXCL1, the receptors Tenascin C, OLR1, IL-18 receptor 1, and growth factors CSF2, CSF3 IL-6, IL1beta and FGF18. The majority of downregulated genes were transcription factors/signalling molecules including ID2. Quantitative real-time RT-PCR analysis confirmed the microarray results for selected genes (CSF3, CX3CL1, FGF18, ID2, SOD2, Tenascin C). CONCLUSIONS: This study reveals a complex gene expression response in HUVEC to anti-beta(2)GPI antibodies with multiple chemokines, pro-inflammatory cytokines, pro-thrombotic and pro-adhesive genes regulated by these antibodies in vitro. Some of these newly identified anti-beta(2)GPI antibody-regulated genes could contribute to the vasculopathy associated with this disease.|
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
beta 2-Glycoprotein I
|Appears in Collections: ||Food Biology, Medical Microbiology and Disinfection Research Group|
|Files in This Item:|
There are no files associated with this item.
|Related articles on PubMed|
Autoantibodies specific to a peptide of β2-glycoprotein I cross-react with TLR4, inducing a proinflammatory phenotype in endothelial cells and monocytes.
Colasanti T, Alessandri C, Capozzi A, Sorice M, Delunardo F, Longo A, Pierdominici M, Conti F, Truglia S, Siracusano A, Valesini G, Ortona E, Margutti P
2012 Oct 18
|See all 102 articles|
All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.