2.50
Hdl Handle:
http://hdl.handle.net/2436/29437
Title:
High diversity of non-human leukocyte antigens in transplant-associated coronary artery disease.
Authors:
Bates, Ruth L.; Frampton, Geoffrey; Rose, Marlene L.; Murphy, John J.
Abstract:
BACKGROUND: Antibodies to endothelial derived non-human leukocyte antigens (HLA) have been associated with transplant (Tx)-associated coronary artery disease (CAD) after cardiac transplantation; however, few have been identified. The aim of this study was to screen a human coronary artery endothelial cell cDNA library with patient sera to establish the diversity and nature of the target antigens. METHODS: A human coronary artery endothelial cell cDNA library was screened with sera from seven long-term cardiac transplant patients with angiographically diagnosed TxCAD and sera from five healthy volunteers. RESULTS: Of the seven patients' sera, five showed reactivity, as did sera from two of the five normal subjects. Eighteen positive cDNA clones were isolated by TxCAD sera; DNA sequence analysis and DNA database searching identified all but one clone; 16 were nuclear or cytoplasmic proteins and 1 of them was the cell surface protein neuropilin 2. Five clones were targeted by normal sera. A different spectrum of reactive clones was identified by the sera of each patient where reactive clones were evident. CONCLUSIONS: A high diversity of non-HLA antigens, probably autoantigens, are involved in the pathogenesis of TxCAD.
Citation:
Transplantation, 75(8): 1347-1350
Publisher:
Lippincott Williams & Wilkins
Journal:
Transplantation
Issue Date:
2003
URI:
http://hdl.handle.net/2436/29437
DOI:
10.1097/01.TP.0000061790.08550.EC
PubMed ID:
12717228
Additional Links:
http://www.transplantjournal.com/pt/re/transplantation/abstract.00007890-200304270-00057.htm
Type:
Article
Language:
en
ISSN:
0041-1337
Appears in Collections:
Centre for Health and Social Care Improvement

Full metadata record

DC FieldValue Language
dc.contributor.authorBates, Ruth L.-
dc.contributor.authorFrampton, Geoffrey-
dc.contributor.authorRose, Marlene L.-
dc.contributor.authorMurphy, John J.-
dc.date.accessioned2008-06-04T09:30:42Z-
dc.date.available2008-06-04T09:30:42Z-
dc.date.issued2003-
dc.identifier.citationTransplantation, 75(8): 1347-1350en
dc.identifier.issn0041-1337-
dc.identifier.pmid12717228-
dc.identifier.doi10.1097/01.TP.0000061790.08550.EC-
dc.identifier.urihttp://hdl.handle.net/2436/29437-
dc.description.abstractBACKGROUND: Antibodies to endothelial derived non-human leukocyte antigens (HLA) have been associated with transplant (Tx)-associated coronary artery disease (CAD) after cardiac transplantation; however, few have been identified. The aim of this study was to screen a human coronary artery endothelial cell cDNA library with patient sera to establish the diversity and nature of the target antigens. METHODS: A human coronary artery endothelial cell cDNA library was screened with sera from seven long-term cardiac transplant patients with angiographically diagnosed TxCAD and sera from five healthy volunteers. RESULTS: Of the seven patients' sera, five showed reactivity, as did sera from two of the five normal subjects. Eighteen positive cDNA clones were isolated by TxCAD sera; DNA sequence analysis and DNA database searching identified all but one clone; 16 were nuclear or cytoplasmic proteins and 1 of them was the cell surface protein neuropilin 2. Five clones were targeted by normal sera. A different spectrum of reactive clones was identified by the sera of each patient where reactive clones were evident. CONCLUSIONS: A high diversity of non-HLA antigens, probably autoantigens, are involved in the pathogenesis of TxCAD.en
dc.language.isoenen
dc.publisherLippincott Williams & Wilkinsen
dc.relation.urlhttp://www.transplantjournal.com/pt/re/transplantation/abstract.00007890-200304270-00057.htmen
dc.subject.meshAdulten
dc.subject.meshAntigensen
dc.subject.meshCoronary Diseaseen
dc.subject.meshCytoplasmen
dc.subject.meshGene Libraryen
dc.subject.meshHeart Transplantationen
dc.subject.meshHumansen
dc.subject.meshMiddle Ageden
dc.subject.meshNeuropilin-2en
dc.subject.meshNuclear Proteinsen
dc.subject.meshProteinsen
dc.subject.meshReference Valuesen
dc.titleHigh diversity of non-human leukocyte antigens in transplant-associated coronary artery disease.en
dc.typeArticleen
dc.identifier.journalTransplantationen
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