Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft.

2.50
Hdl Handle:
http://hdl.handle.net/2436/29433
Title:
Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft.
Authors:
Tin, Mandy; Cho, Chi-Hin; Chan, Kelvin C.; James, Anthony; Ko, Joshua
Abstract:
Astragalus memebranaceus is used as immunomodulating agent in treating immunodeficiency diseases and to alleviate the adverse effects of chemotherapeutic drugs. In recent years, it has been proposed that Astragalus may possess anti-tumorigenic potential in certain cancer cell types. In this study, the anti-carcinogenic effects of Astragalus saponin extract were investigated in HT-29 human colon cancer cells and tumor xenograft. Our findings have shown that Astragalus saponins (AST) inhibit cell proliferation through accumulation in S phase and G2/M arrest, with concomitant suppression of p21 expression and inhibition of cyclin-dependent kinase activity. Besides, AST promotes apoptosis in HT-29 cells through caspase 3 activation and poly(ADP-ribose) polymerase cleavage, which is indicated by DNA fragmentation and nuclear chromatin condensation. Nevertheless, we also demonstrate the anti-tumorigenic effects of AST in vivo, of which the reduction of tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable with that produced by the conventional chemotherapeutic drug 5-fluorouracil (5-FU). In addition, the side effects (body weight drop and mortality) associated with the drug combo 5-FU and oxaliplatin are not induced by AST. These results indicate that AST could be an effective chemotherapeutic agent in colon cancer treatment, which might also be used as an adjuvant in combination with other orthodox chemotherapeutic drugs to reduce the side effects of the latter compounds.
Citation:
Carcinogenesis, 28(6): 1347-1355
Publisher:
Oxford Journals
Journal:
Carcinogenesis
Issue Date:
2007
URI:
http://hdl.handle.net/2436/29433
DOI:
10.1093/carcin/bgl238
PubMed ID:
17148504
Additional Links:
http://carcin.oxfordjournals.org/cgi/content/full/28/6/1347
Type:
Article
Language:
en
ISSN:
0143-3334
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorTin, Mandy-
dc.contributor.authorCho, Chi-Hin-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorJames, Anthony-
dc.contributor.authorKo, Joshua-
dc.date.accessioned2008-06-04T08:52:09Z-
dc.date.available2008-06-04T08:52:09Z-
dc.date.issued2007-
dc.identifier.citationCarcinogenesis, 28(6): 1347-1355en
dc.identifier.issn0143-3334-
dc.identifier.pmid17148504-
dc.identifier.doi10.1093/carcin/bgl238-
dc.identifier.urihttp://hdl.handle.net/2436/29433-
dc.description.abstractAstragalus memebranaceus is used as immunomodulating agent in treating immunodeficiency diseases and to alleviate the adverse effects of chemotherapeutic drugs. In recent years, it has been proposed that Astragalus may possess anti-tumorigenic potential in certain cancer cell types. In this study, the anti-carcinogenic effects of Astragalus saponin extract were investigated in HT-29 human colon cancer cells and tumor xenograft. Our findings have shown that Astragalus saponins (AST) inhibit cell proliferation through accumulation in S phase and G2/M arrest, with concomitant suppression of p21 expression and inhibition of cyclin-dependent kinase activity. Besides, AST promotes apoptosis in HT-29 cells through caspase 3 activation and poly(ADP-ribose) polymerase cleavage, which is indicated by DNA fragmentation and nuclear chromatin condensation. Nevertheless, we also demonstrate the anti-tumorigenic effects of AST in vivo, of which the reduction of tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable with that produced by the conventional chemotherapeutic drug 5-fluorouracil (5-FU). In addition, the side effects (body weight drop and mortality) associated with the drug combo 5-FU and oxaliplatin are not induced by AST. These results indicate that AST could be an effective chemotherapeutic agent in colon cancer treatment, which might also be used as an adjuvant in combination with other orthodox chemotherapeutic drugs to reduce the side effects of the latter compounds.en
dc.language.isoenen
dc.publisherOxford Journalsen
dc.relation.urlhttp://carcin.oxfordjournals.org/cgi/content/full/28/6/1347en
dc.subject.meshAnimalsen
dc.subject.meshAntineoplastic Agentsen
dc.subject.meshApoptosisen
dc.subject.meshAstragalus Planten
dc.subject.meshColonic Neoplasmsen
dc.subject.meshHT29 Cellsen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshMice, Nudeen
dc.subject.meshSaponinsen
dc.subject.meshTransplantation, Heterologousen
dc.subject.meshXenograft Model Antitumor Assaysen
dc.titleAstragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft.en
dc.typeArticleen
dc.identifier.journalCarcinogenesisen
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