University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Pharmacy and Natural Products Research Group > Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/28652
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis.
Authors: Lu, Guang-Hua
Chan, Kelvin C.
Leung, Kelvin Sze-Yin
Chan, Chi-Leung
Zhao, Zhong-Zhen
Jiang, Zhi-Hong
Citation: Journal of Chromatography A, 1068 (2): 209-219
Journal: Journal of Chromatography A
Issue Date: 2005
URI: http://hdl.handle.net/2436/28652
DOI: 10.1016/j.chroma.2005.01.082
PubMed ID: 15830926
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4FGX5C2-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=b7ed7f8e598bcec55073d612da17703a
Abstract: Activity of Chinese Danggui (DG), the processed root of Angelica sinensis (Oliv.) Diels, is linked to the ferulic acid content but the stability of ferulic acid during extraction for medicinal use is not known. The stabilities of ferulic acid and coniferyl ferulate were evaluated in the extracts of DG using a variety of extraction solvents. These included various combinations and proportions of methanol, water, formic acid, 1 M aqueous hydrochloric acid and 2% sodium hydrogen carbonate (NaHCO3) in water. Coniferyl ferulate was found liable to hydrolyze into ferulic acid in neutral, strongly acidic and basic solvents, where heat and water could facilitate this hydrolysis. However, the hydrolysis was relatively resisted in weakly organic acid. Based on the stability evaluation, two new terms, namely: free ferulic acid and total ferulic acid, were suggested and defined. Free ferulic acid refers to the natural content of ferulic acid in herbs. Total ferulic acid means the sum of free ferulic acid plus the amount of related hydrolyzed components. Meanwhile, the high-performance liquid chromatographic (HPLC) method was developed to assay free ferulic acid and total ferulic acid in DG using methanol-formic acid (95:5) and methanol-2% NaHCO3 in water (95:5) as extraction solvents, respectively. Ten DG samples were investigated on their contents of free and total ferulic acid. The results indicated that the amount variety of free ferulic acid was larger than that of their counterparts, and the ratio of total ferulic acid to free ferulic acid was 4.07 +/- 2.73 (mean +/- SD, n = 10). The chemical assay of DG using total ferulic acid content would be a better choice to assess the herbal quality and was recommended.
Type: Article
Language: en
Keywords: Ferulic Acid
Coniferyl Ferulate
Plants, Medicinal
HPLC
Pharmaceutical analysis
MeSH: Angelica sinensis
Chromatography, High Pressure Liquid
Coumaric Acids
Drug Stability
Drugs, Chinese Herbal
Plant Roots
Quality Control
Reproducibility of Results
ISSN: 0021-9673
Appears in Collections: Pharmacy and Natural Products Research Group

Files in This Item:

There are no files associated with this item.



Related articles on PubMed
bullet
bullet
bullet
bullet
[Study on quality standard of oil-processed Radix Angelica sinensis].
Yang HS, Hua YL, Guo YS, Du TX, Yang XH, Qinh L, Qu YL, Wei YM
2010 Jun
bullet
See all 219 articles

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies