Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis.

2.50
Hdl Handle:
http://hdl.handle.net/2436/28652
Title:
Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis.
Authors:
Lu, Guang-Hua; Chan, Kelvin C.; Leung, Kelvin Sze-Yin; Chan, Chi-Leung; Zhao, Zhong-Zhen; Jiang, Zhi-Hong
Abstract:
Activity of Chinese Danggui (DG), the processed root of Angelica sinensis (Oliv.) Diels, is linked to the ferulic acid content but the stability of ferulic acid during extraction for medicinal use is not known. The stabilities of ferulic acid and coniferyl ferulate were evaluated in the extracts of DG using a variety of extraction solvents. These included various combinations and proportions of methanol, water, formic acid, 1 M aqueous hydrochloric acid and 2% sodium hydrogen carbonate (NaHCO3) in water. Coniferyl ferulate was found liable to hydrolyze into ferulic acid in neutral, strongly acidic and basic solvents, where heat and water could facilitate this hydrolysis. However, the hydrolysis was relatively resisted in weakly organic acid. Based on the stability evaluation, two new terms, namely: free ferulic acid and total ferulic acid, were suggested and defined. Free ferulic acid refers to the natural content of ferulic acid in herbs. Total ferulic acid means the sum of free ferulic acid plus the amount of related hydrolyzed components. Meanwhile, the high-performance liquid chromatographic (HPLC) method was developed to assay free ferulic acid and total ferulic acid in DG using methanol-formic acid (95:5) and methanol-2% NaHCO3 in water (95:5) as extraction solvents, respectively. Ten DG samples were investigated on their contents of free and total ferulic acid. The results indicated that the amount variety of free ferulic acid was larger than that of their counterparts, and the ratio of total ferulic acid to free ferulic acid was 4.07 +/- 2.73 (mean +/- SD, n = 10). The chemical assay of DG using total ferulic acid content would be a better choice to assess the herbal quality and was recommended.
Citation:
Journal of Chromatography A, 1068 (2): 209-219
Journal:
Journal of Chromatography A
Issue Date:
2005
URI:
http://hdl.handle.net/2436/28652
DOI:
10.1016/j.chroma.2005.01.082
PubMed ID:
15830926
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4FGX5C2-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=b7ed7f8e598bcec55073d612da17703a
Type:
Article
Language:
en
ISSN:
0021-9673
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Guang-Hua-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorLeung, Kelvin Sze-Yin-
dc.contributor.authorChan, Chi-Leung-
dc.contributor.authorZhao, Zhong-Zhen-
dc.contributor.authorJiang, Zhi-Hong-
dc.date.accessioned2008-05-28T13:32:00Z-
dc.date.available2008-05-28T13:32:00Z-
dc.date.issued2005-
dc.identifier.citationJournal of Chromatography A, 1068 (2): 209-219en
dc.identifier.issn0021-9673-
dc.identifier.pmid15830926-
dc.identifier.doi10.1016/j.chroma.2005.01.082-
dc.identifier.urihttp://hdl.handle.net/2436/28652-
dc.description.abstractActivity of Chinese Danggui (DG), the processed root of Angelica sinensis (Oliv.) Diels, is linked to the ferulic acid content but the stability of ferulic acid during extraction for medicinal use is not known. The stabilities of ferulic acid and coniferyl ferulate were evaluated in the extracts of DG using a variety of extraction solvents. These included various combinations and proportions of methanol, water, formic acid, 1 M aqueous hydrochloric acid and 2% sodium hydrogen carbonate (NaHCO3) in water. Coniferyl ferulate was found liable to hydrolyze into ferulic acid in neutral, strongly acidic and basic solvents, where heat and water could facilitate this hydrolysis. However, the hydrolysis was relatively resisted in weakly organic acid. Based on the stability evaluation, two new terms, namely: free ferulic acid and total ferulic acid, were suggested and defined. Free ferulic acid refers to the natural content of ferulic acid in herbs. Total ferulic acid means the sum of free ferulic acid plus the amount of related hydrolyzed components. Meanwhile, the high-performance liquid chromatographic (HPLC) method was developed to assay free ferulic acid and total ferulic acid in DG using methanol-formic acid (95:5) and methanol-2% NaHCO3 in water (95:5) as extraction solvents, respectively. Ten DG samples were investigated on their contents of free and total ferulic acid. The results indicated that the amount variety of free ferulic acid was larger than that of their counterparts, and the ratio of total ferulic acid to free ferulic acid was 4.07 +/- 2.73 (mean +/- SD, n = 10). The chemical assay of DG using total ferulic acid content would be a better choice to assess the herbal quality and was recommended.en
dc.language.isoenen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4FGX5C2-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=b7ed7f8e598bcec55073d612da17703a-
dc.subjectFerulic Aciden
dc.subjectConiferyl Ferulateen
dc.subjectPlants, Medicinalen
dc.subjectHPLCen
dc.subjectPharmaceutical analysisen
dc.subject.meshAngelica sinensisen
dc.subject.meshChromatography, High Pressure Liquiden
dc.subject.meshCoumaric Acidsen
dc.subject.meshDrug Stabilityen
dc.subject.meshDrugs, Chinese Herbalen
dc.subject.meshPlant Rootsen
dc.subject.meshQuality Controlen
dc.subject.meshReproducibility of Resultsen
dc.titleAssay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis.en
dc.typeArticleen
dc.identifier.journalJournal of Chromatography Aen

Related articles on PubMed

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.