Cloning of a novel gene encoding a C2H2 zinc finger protein that alleviates sensitivity to abiotic stresses in Aspergillus nidulans

2.50
Hdl Handle:
http://hdl.handle.net/2436/22192
Title:
Cloning of a novel gene encoding a C2H2 zinc finger protein that alleviates sensitivity to abiotic stresses in Aspergillus nidulans
Authors:
O'Neil, John D.; Bugno, Marcin; Stanley, Michele S.; Barham-Morris, Julia B.; Woodcock, Nicola A.; Clement, Darren J.; Clipson, Nicholas J. W.; Whitehead, Michael P.; Fincham, Daron A.; Hooley, Paul
Abstract:
We report the cloning and sequencing of a DNA fragment encoding a putative C2H2 zinc finger protein from Aspergillus nidulans. The gene was isolated by complementation cloning of a salt sensitive phenotype of the A. nidulans sltAl mutant. A 3.8 kb Pst I fragment that restored wild type salt tolerance contained one large open reading frame of 2202 bp. The predicted protein (StzA) from this reading frame comprises 698 amino acids and has three Zinc fingers along with a putative transcriptional activation domain rich in acidic amino acids. The corresponding sequence from a sltAl mutant contains a premature STOP codon resulting in loss of the putative transcriptional activator in the C-terminal region. The Zinc fingers show conserved motifs with a number of transcription factors including CreA from A. nidulans and the human Wilm's tumour susceptibility protein WT-1.
Citation:
Mycological Research, 106(4): 491-498
Publisher:
Elsevier Science Direct
Journal:
Mycological Research
Issue Date:
2002
URI:
http://hdl.handle.net/2436/22192
DOI:
10.1017/S0953756202005701
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7XMR-4RR97B2-J&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=af34e34456d8e3a7f9b82176773566fd
Type:
Article
Language:
en
ISSN:
09537562; 14698102
Appears in Collections:
Cancer Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorO'Neil, John D.-
dc.contributor.authorBugno, Marcin-
dc.contributor.authorStanley, Michele S.-
dc.contributor.authorBarham-Morris, Julia B.-
dc.contributor.authorWoodcock, Nicola A.-
dc.contributor.authorClement, Darren J.-
dc.contributor.authorClipson, Nicholas J. W.-
dc.contributor.authorWhitehead, Michael P.-
dc.contributor.authorFincham, Daron A.-
dc.contributor.authorHooley, Paul-
dc.date.accessioned2008-04-03T08:59:26Z-
dc.date.available2008-04-03T08:59:26Z-
dc.date.issued2002-
dc.identifier.citationMycological Research, 106(4): 491-498en
dc.identifier.issn09537562-
dc.identifier.issn14698102-
dc.identifier.doi10.1017/S0953756202005701-
dc.identifier.urihttp://hdl.handle.net/2436/22192-
dc.description.abstractWe report the cloning and sequencing of a DNA fragment encoding a putative C2H2 zinc finger protein from Aspergillus nidulans. The gene was isolated by complementation cloning of a salt sensitive phenotype of the A. nidulans sltAl mutant. A 3.8 kb Pst I fragment that restored wild type salt tolerance contained one large open reading frame of 2202 bp. The predicted protein (StzA) from this reading frame comprises 698 amino acids and has three Zinc fingers along with a putative transcriptional activation domain rich in acidic amino acids. The corresponding sequence from a sltAl mutant contains a premature STOP codon resulting in loss of the putative transcriptional activator in the C-terminal region. The Zinc fingers show conserved motifs with a number of transcription factors including CreA from A. nidulans and the human Wilm's tumour susceptibility protein WT-1.en
dc.language.isoenen
dc.publisherElsevier Science Directen
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7XMR-4RR97B2-J&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=af34e34456d8e3a7f9b82176773566fden
dc.subjectC2H2en
dc.subjectZinc fingersen
dc.subjectAspergillus nidulansen
dc.subjectProteinsen
dc.subjectAmino Acidsen
dc.subjectAbiotic stressen
dc.titleCloning of a novel gene encoding a C2H2 zinc finger protein that alleviates sensitivity to abiotic stresses in Aspergillus nidulansen
dc.typeArticleen
dc.identifier.journalMycological Researchen
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