ICAM-1 expression and leukocyte behavior in the microcirculation of chronically ischemic rat skeletal muscles.

2.50
Hdl Handle:
http://hdl.handle.net/2436/20979
Title:
ICAM-1 expression and leukocyte behavior in the microcirculation of chronically ischemic rat skeletal muscles.
Authors:
Anderson, Stephen I.; Shiner, Ruth; Brown, Margaret D.; Hudlicka, Olga
Abstract:
In muscle microcirculation, short periods of ischemia followed by reperfusion are known to upregulate leukocyte and endothelial adhesion molecules, but little is known about leukocyte adherence and ICAM-1 expression during chronic ischemia or any likely effect of muscle activity which is recommended in chronic ischemia due to peripheral arterial disease. Leukocyte rolling and stationary adhesion were observed in post-capillary venules in ischemic and contralateral rat extensor digitorum longus (EDL) muscles 3 and 7 days after unilateral ligation of the common iliac artery and in 3-day ischemic EDLs that were electrically stimulated on days 1 and 2 post-ligation (7 x 15 min per day). ICAM-1 was localized immunohistochemically to venular vessels in all muscles. Following ligation, use of the ischemic leg was observed to be restricted for the first 3 days, returning to normal by 7 days. After 3 days, leukocyte rolling/adherence and ICAM-1 expression were no different in ischemic than control muscles, but all were increased in contralateral muscles. In ischemic muscles, electrical stimulation doubled the numbers of rolling leukocytes and upregulated ICAM-1 expression. After 7 days, increased muscle activity as a result of natural movement also resulted in greater ICAM-1 expression, a 4- to 5-fold increase in rolling leukocyte numbers and a 3-fold increase in stationary adherent leukocytes. Chronic ischemia thus increases ICAM-1 and leukocyte adherence in muscle microcirculation only when combined with contractile activity. Post-capillary venular endothelium may be modified by muscle acidosis when contractions are performed under low flow conditions or by changes in rheological (shear force) factors.
Citation:
Microvascular Research, 71(3): 205-211
Publisher:
Elsevier
Journal:
Microvascular Research
Issue Date:
2006
URI:
http://hdl.handle.net/2436/20979
DOI:
10.1016/j.mvr.2006.03.003
PubMed ID:
16647725
Additional Links:
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WN8-4JVSX0W-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=cefd11f96662be00ed42abaab972eaa7
Type:
Article
Language:
en
ISSN:
0026-2862
Appears in Collections:
Diabetes, Physiology and Molecular Medicine Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorAnderson, Stephen I.-
dc.contributor.authorShiner, Ruth-
dc.contributor.authorBrown, Margaret D.-
dc.contributor.authorHudlicka, Olga-
dc.date.accessioned2008-03-18T11:16:42Z-
dc.date.available2008-03-18T11:16:42Z-
dc.date.issued2006-
dc.identifier.citationMicrovascular Research, 71(3): 205-211en
dc.identifier.issn0026-2862-
dc.identifier.pmid16647725-
dc.identifier.doi10.1016/j.mvr.2006.03.003-
dc.identifier.urihttp://hdl.handle.net/2436/20979-
dc.description.abstractIn muscle microcirculation, short periods of ischemia followed by reperfusion are known to upregulate leukocyte and endothelial adhesion molecules, but little is known about leukocyte adherence and ICAM-1 expression during chronic ischemia or any likely effect of muscle activity which is recommended in chronic ischemia due to peripheral arterial disease. Leukocyte rolling and stationary adhesion were observed in post-capillary venules in ischemic and contralateral rat extensor digitorum longus (EDL) muscles 3 and 7 days after unilateral ligation of the common iliac artery and in 3-day ischemic EDLs that were electrically stimulated on days 1 and 2 post-ligation (7 x 15 min per day). ICAM-1 was localized immunohistochemically to venular vessels in all muscles. Following ligation, use of the ischemic leg was observed to be restricted for the first 3 days, returning to normal by 7 days. After 3 days, leukocyte rolling/adherence and ICAM-1 expression were no different in ischemic than control muscles, but all were increased in contralateral muscles. In ischemic muscles, electrical stimulation doubled the numbers of rolling leukocytes and upregulated ICAM-1 expression. After 7 days, increased muscle activity as a result of natural movement also resulted in greater ICAM-1 expression, a 4- to 5-fold increase in rolling leukocyte numbers and a 3-fold increase in stationary adherent leukocytes. Chronic ischemia thus increases ICAM-1 and leukocyte adherence in muscle microcirculation only when combined with contractile activity. Post-capillary venular endothelium may be modified by muscle acidosis when contractions are performed under low flow conditions or by changes in rheological (shear force) factors.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WN8-4JVSX0W-1&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=cefd11f96662be00ed42abaab972eaa7en
dc.subjectPost-capillary venulesen
dc.subjectLeukocyte rolling and adhesionen
dc.subjectMuscle ischemiaen
dc.subjectMuscle activityen
dc.subject.meshAnimalsen
dc.subject.meshBlood Flow Velocityen
dc.subject.meshCell Adhesionen
dc.subject.meshChronic Diseaseen
dc.subject.meshElectric Stimulationen
dc.subject.meshIntercellular Adhesion Molecule-1en
dc.subject.meshIschemiaen
dc.subject.meshLeukocyte Counten
dc.subject.meshLeukocyte Rollingen
dc.subject.meshLeukocytesen
dc.subject.meshMaleen
dc.subject.meshMicrocirculationen
dc.subject.meshMuscle Contractionen
dc.subject.meshMuscle, Skeletalen
dc.subject.meshRatsen
dc.subject.meshRats, Wistaren
dc.titleICAM-1 expression and leukocyte behavior in the microcirculation of chronically ischemic rat skeletal muscles.en
dc.typeArticleen
dc.identifier.journalMicrovascular Researchen

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