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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Diabetes, Physiology and Molecular Medicine Research Group > Leptin decreases apoptosis and alters BCL-2 : Bax ratio in clonal rodent pancreatic beta-cells.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/20393
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Title: Leptin decreases apoptosis and alters BCL-2 : Bax ratio in clonal rodent pancreatic beta-cells.
Authors: Brown, James E. P.
Dunmore, Simon J.
Citation: Diabetes/Metabolism Research Reviews, 23(6): 497-502
Publisher: Wiley Interscience
Issue Date: 2007
URI: http://hdl.handle.net/2436/20393
DOI: 10.1002/dmrr.726
PubMed ID: 17318810
Additional Links: http://www3.interscience.wiley.com/cgi-bin/abstract/114123262/
Abstract: AIMS/HYPOTHESIS: The adipocyte derived peptide hormone leptin is known to regulate apoptosis and cell viability in several cells and tissues, as well as having several pancreatic islet beta-cell specific effects such as inhibition of glucose-stimulated insulin secretion. This study investigated the effects of leptin upon apoptosis induced by serum depletion and on expression of the apoptotic regulators B-cell leukaemia 2 gene product (BCL-2) and BCL2-associated X protein (Bax) in the glucose-responsive BRIN-BD11 beta-cell line. METHODS: BRIN-BD11 cells were cultured in RPMI 1640 and subsequently serum depleted +/- leptin (10 and 50 ng/mL) for 24 h. Cell viability and apoptosis were measured using a modified MTS assay and TUNEL/YO-PRO-1 assays, respectively. BCL-2 and Bax expression were measured by real-time PCR and Western blotting. RESULTS: Leptin caused a reduction in serum-depleted apoptosis, although it failed to have any effect on the overall cell viability, causing a 68% shift from apoptosis to necrosis. Leptin significantly increased the level of BCL-2 mRNA expression (150% compared to serum depletion alone), without altering Bax mRNA expression. At the protein level, leptin increased BCL-2 and decreased Bax, altering the BCL-2 : Bax ratio. CONCLUSIONS: We conclude that leptin reduces apoptosis in beta-cells at physiological concentrations, possibly via its ability to up-regulate BCL-2 and Bax expression.
Type: Article
Language: en
Keywords: Leptin
Apoptosis
Beta-cell
BCL-2
Bax
MeSH: Animals
Apoptosis
Cell Line
Culture Media, Serum-Free
Insulin-Secreting Cells
Leptin
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger
Rats
bcl-2-Associated X Protein
ISSN: 1520-7552
Appears in Collections: Diabetes, Physiology and Molecular Medicine Research Group

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