Identification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.

2.50
Hdl Handle:
http://hdl.handle.net/2436/17052
Title:
Identification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.
Authors:
Baldwin, Timothy C.; Handford, Michael G.; Yuseff, Maria-Isabel; Orellana, Ariel; Dupree, Paul
Abstract:
Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.
Citation:
The Plant Cell, 13(10): 2283-2295
Publisher:
American Society of Plant Biologists
Issue Date:
2001
URI:
http://hdl.handle.net/2436/17052
DOI:
10.1105/tpc.13.10.2283
PubMed ID:
11595802
Additional Links:
http://www.plantcell.org/cgi/content/full/13/10/2283
Type:
Article
Language:
en
Description:
Metadata only, Full text available at links above.
ISSN:
1040-4651
Appears in Collections:
Agriculture Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorBaldwin, Timothy C.-
dc.contributor.authorHandford, Michael G.-
dc.contributor.authorYuseff, Maria-Isabel-
dc.contributor.authorOrellana, Ariel-
dc.contributor.authorDupree, Paul-
dc.date.accessioned2008-01-29T14:30:31Z-
dc.date.available2008-01-29T14:30:31Z-
dc.date.issued2001-
dc.identifier.citationThe Plant Cell, 13(10): 2283-2295en
dc.identifier.issn1040-4651-
dc.identifier.pmid11595802-
dc.identifier.doi10.1105/tpc.13.10.2283-
dc.identifier.urihttp://hdl.handle.net/2436/17052-
dc.descriptionMetadata only, Full text available at links above.en
dc.description.abstractTransport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.en
dc.language.isoenen
dc.publisherAmerican Society of Plant Biologistsen
dc.relation.urlhttp://www.plantcell.org/cgi/content/full/13/10/2283en
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshArabidopsisen
dc.subject.meshArabidopsis Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshCarrier Proteinsen
dc.subject.meshDNA Primersen
dc.subject.meshGlycosylationen
dc.subject.meshGolgi Apparatusen
dc.subject.meshIntracellular Membranesen
dc.subject.meshMembrane Transport Proteinsen
dc.subject.meshMicroscopy, Confocalen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshProtein Conformationen
dc.subject.meshSequence Alignmenten
dc.subject.meshSequence Homology, Amino Aciden
dc.titleIdentification and characterization of GONST1, a golgi-localized GDP-mannose transporter in Arabidopsis.en
dc.typeArticleen

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