Developmental regulation of mannan, arabinogalactan-protein and pectic epitopes in pistils of Vicia faba (faba bean)

2.50
Hdl Handle:
http://hdl.handle.net/2436/17033
Title:
Developmental regulation of mannan, arabinogalactan-protein and pectic epitopes in pistils of Vicia faba (faba bean)
Authors:
Chen, Wen; Stoddard, Fred L.; Baldwin, Timothy C.
Abstract:
Cell walls of the stigma and style are important zones for cell‐cell recognition, nutrition, guidance, and protection of the pollen tube along the transmitting tract. The objective of this study was to investigate the modulation of selected cell wall epitopes during pistil development in the crop species Vicia faba L. (faba bean). An immunocytochemical investigation of pistil development was performed using a panel of four anti–cell wall/plasma membrane antibodies at both light and electron microscope levels during the 4 days leading up to anthesis. The selected antibodies recognized mannan‐containing polysaccharides (antimannan antisera), β‐1,4‐galactan (LM5), α‐1,5‐l‐arabinan (LM6), and arabinogalactan/arabinogalactan‐protein (AG/AGP) epitopes, with GlcpUA‐β‐1,→3‐d‐GalpUA‐α‐1,→2‐l‐Rha trisaccharide motifs (JIM13). Mannan accumulation was shown to be associated with the formation and maturation of sclerenchyma cells and xylem elements. The β‐1,4‐galactan and α‐1,5‐l‐arabinan epitopes were highly expressed in the walls of parenchyma and epidermis in a developmentally regulated manner. AG/AGP epitopes were particularly abundant in the stigma and cells lining the stylar canal, together with the α‐1,5‐l‐arabinan epitope recognized by LM6. These data suggest an association of AGs/AGPs with pollen tube growth, of mannan with strengthening and stiffening the pistil, and of β‐1,4‐galactan and α‐1,5‐l‐arabinan components with tissue flexibility within the pistil.
Citation:
International Journal of Plant Sciences, 167(5): 919-932
Publisher:
University of Chicago Press
Issue Date:
2006
URI:
http://hdl.handle.net/2436/17033
DOI:
10.1086/506330
Additional Links:
http://www.journals.uchicago.edu/doi/abs/10.1086/506330
Type:
Article
Language:
en
Description:
Metadata only
ISSN:
10585893; 15375315
Appears in Collections:
Agriculture Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Wen-
dc.contributor.authorStoddard, Fred L.-
dc.contributor.authorBaldwin, Timothy C.-
dc.date.accessioned2008-01-29T14:55:33Z-
dc.date.available2008-01-29T14:55:33Z-
dc.date.issued2006-
dc.identifier.citationInternational Journal of Plant Sciences, 167(5): 919-932en
dc.identifier.issn10585893-
dc.identifier.issn15375315-
dc.identifier.doi10.1086/506330-
dc.identifier.urihttp://hdl.handle.net/2436/17033-
dc.descriptionMetadata onlyen
dc.description.abstractCell walls of the stigma and style are important zones for cell‐cell recognition, nutrition, guidance, and protection of the pollen tube along the transmitting tract. The objective of this study was to investigate the modulation of selected cell wall epitopes during pistil development in the crop species Vicia faba L. (faba bean). An immunocytochemical investigation of pistil development was performed using a panel of four anti–cell wall/plasma membrane antibodies at both light and electron microscope levels during the 4 days leading up to anthesis. The selected antibodies recognized mannan‐containing polysaccharides (antimannan antisera), β‐1,4‐galactan (LM5), α‐1,5‐l‐arabinan (LM6), and arabinogalactan/arabinogalactan‐protein (AG/AGP) epitopes, with GlcpUA‐β‐1,→3‐d‐GalpUA‐α‐1,→2‐l‐Rha trisaccharide motifs (JIM13). Mannan accumulation was shown to be associated with the formation and maturation of sclerenchyma cells and xylem elements. The β‐1,4‐galactan and α‐1,5‐l‐arabinan epitopes were highly expressed in the walls of parenchyma and epidermis in a developmentally regulated manner. AG/AGP epitopes were particularly abundant in the stigma and cells lining the stylar canal, together with the α‐1,5‐l‐arabinan epitope recognized by LM6. These data suggest an association of AGs/AGPs with pollen tube growth, of mannan with strengthening and stiffening the pistil, and of β‐1,4‐galactan and α‐1,5‐l‐arabinan components with tissue flexibility within the pistil.en
dc.language.isoenen
dc.publisherUniversity of Chicago Pressen
dc.relation.urlhttp://www.journals.uchicago.edu/doi/abs/10.1086/506330en
dc.subjectFaba beanen
dc.subjectOpen styleen
dc.subjectArabinogalactan-proteinen
dc.subjectβ‐galactanen
dc.subjectMannanen
dc.subjectα‐arabinanen
dc.titleDevelopmental regulation of mannan, arabinogalactan-protein and pectic epitopes in pistils of Vicia faba (faba bean)en
dc.typeArticleen
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