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Wolverhampton Intellectual Repository and E-Theses > School of Applied Sciences > Research Centre in Applied Sciences  > Agriculture Research Group > DcAGP1, a secreted arabinogalactan protein, is related to a family of basic proline-rich proteins.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/17032
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Title: DcAGP1, a secreted arabinogalactan protein, is related to a family of basic proline-rich proteins.
Authors: Baldwin, Timothy C.
Domingo, Concha
Schindler, Thomas
Seetharaman, Gouri
Stacey, Nicola
Roberts, Keith
Citation: Plant molecular biology, 45(4): 421-435
Publisher: Springer Verlag
Issue Date: 2001
URI: http://hdl.handle.net/2436/17032
DOI: 10.1023/A:1010637426934
PubMed ID: 11352461
Additional Links: http://www.springerlink.com/content/t2625751268010wm/
Abstract: A cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) secreted aucus carota (carrot) cells in liquid culture was isolated. This cDNA, DcAGP1, encodes a new class of non-classical' AGP with strong similarity to a family of basic proline-rich proteins. The protein is rich in proline (17%), alanine (10%) and lysine (11%) and contains four distinct domains: a signal peptide, a proline-rich domain, a histidine-rich basic domain and a cysteine-containing 'PAC' domain that is found in a range of other cell wall proteins. The protein contains several sequence motifs found in otherwise unrelated cell wall proteins, but also displays some unique features. Northern blot analyses show that while the DcAGP1 transcript is abundant in the suspension-culture cells from which the AGP was obtained; in carrot seedlings the gene is only expressed at low levels in the roots and it is neither wound- nor stress-inducible. Furthermore, northern and western blot analyses demonstrate that the core polypeptide of DcAGP1 is differentially glycosylated in two different carrot suspension cultures. The unusual features of the protein sequence suggest that the DcAGP1 protein is a member of a family of basic proline-rich proteins defined by the C-terminal PAC domain, and the possible function(s) of the DcAGP1 protein is considered in the light of current views on AGP structure and function.
Type: Article
Language: en
Description: Metadata only. Full text available at links above.
MeSH: Amino Acid Sequence
Base Sequence
Blotting, Northern
DNA, Complementary
Daucus carota
Gene Expression Regulation, Plant
Molecular Sequence Data
Mucoproteins
Peptides
Phylogeny
Plant Proteins
Plants
RNA, Plant
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
ISSN: 0167-4412
Appears in Collections: Agriculture Research Group

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