DcAGP1, a secreted arabinogalactan protein, is related to a family of basic proline-rich proteins.

2.50
Hdl Handle:
http://hdl.handle.net/2436/17032
Title:
DcAGP1, a secreted arabinogalactan protein, is related to a family of basic proline-rich proteins.
Authors:
Baldwin, Timothy C.; Domingo, Concha; Schindler, Thomas; Seetharaman, Gouri; Stacey, Nicola; Roberts, Keith
Abstract:
A cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) secreted aucus carota (carrot) cells in liquid culture was isolated. This cDNA, DcAGP1, encodes a new class of non-classical' AGP with strong similarity to a family of basic proline-rich proteins. The protein is rich in proline (17%), alanine (10%) and lysine (11%) and contains four distinct domains: a signal peptide, a proline-rich domain, a histidine-rich basic domain and a cysteine-containing 'PAC' domain that is found in a range of other cell wall proteins. The protein contains several sequence motifs found in otherwise unrelated cell wall proteins, but also displays some unique features. Northern blot analyses show that while the DcAGP1 transcript is abundant in the suspension-culture cells from which the AGP was obtained; in carrot seedlings the gene is only expressed at low levels in the roots and it is neither wound- nor stress-inducible. Furthermore, northern and western blot analyses demonstrate that the core polypeptide of DcAGP1 is differentially glycosylated in two different carrot suspension cultures. The unusual features of the protein sequence suggest that the DcAGP1 protein is a member of a family of basic proline-rich proteins defined by the C-terminal PAC domain, and the possible function(s) of the DcAGP1 protein is considered in the light of current views on AGP structure and function.
Citation:
Plant molecular biology, 45(4): 421-435
Publisher:
Springer Verlag
Issue Date:
2001
URI:
http://hdl.handle.net/2436/17032
DOI:
10.1023/A:1010637426934
PubMed ID:
11352461
Additional Links:
http://www.springerlink.com/content/t2625751268010wm/
Type:
Article
Language:
en
Description:
Metadata only. Full text available at links above.
ISSN:
0167-4412
Appears in Collections:
Agriculture Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorBaldwin, Timothy C.-
dc.contributor.authorDomingo, Concha-
dc.contributor.authorSchindler, Thomas-
dc.contributor.authorSeetharaman, Gouri-
dc.contributor.authorStacey, Nicola-
dc.contributor.authorRoberts, Keith-
dc.date.accessioned2008-01-29T14:22:49Z-
dc.date.available2008-01-29T14:22:49Z-
dc.date.issued2001-
dc.identifier.citationPlant molecular biology, 45(4): 421-435en
dc.identifier.issn0167-4412-
dc.identifier.pmid11352461-
dc.identifier.doi10.1023/A:1010637426934-
dc.identifier.urihttp://hdl.handle.net/2436/17032-
dc.descriptionMetadata only. Full text available at links above.en
dc.description.abstractA cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) secreted aucus carota (carrot) cells in liquid culture was isolated. This cDNA, DcAGP1, encodes a new class of non-classical' AGP with strong similarity to a family of basic proline-rich proteins. The protein is rich in proline (17%), alanine (10%) and lysine (11%) and contains four distinct domains: a signal peptide, a proline-rich domain, a histidine-rich basic domain and a cysteine-containing 'PAC' domain that is found in a range of other cell wall proteins. The protein contains several sequence motifs found in otherwise unrelated cell wall proteins, but also displays some unique features. Northern blot analyses show that while the DcAGP1 transcript is abundant in the suspension-culture cells from which the AGP was obtained; in carrot seedlings the gene is only expressed at low levels in the roots and it is neither wound- nor stress-inducible. Furthermore, northern and western blot analyses demonstrate that the core polypeptide of DcAGP1 is differentially glycosylated in two different carrot suspension cultures. The unusual features of the protein sequence suggest that the DcAGP1 protein is a member of a family of basic proline-rich proteins defined by the C-terminal PAC domain, and the possible function(s) of the DcAGP1 protein is considered in the light of current views on AGP structure and function.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.relation.urlhttp://www.springerlink.com/content/t2625751268010wm/en
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshBase Sequenceen
dc.subject.meshBlotting, Northernen
dc.subject.meshDNA, Complementaryen
dc.subject.meshDaucus carotaen
dc.subject.meshGene Expression Regulation, Planten
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMucoproteinsen
dc.subject.meshPeptidesen
dc.subject.meshPhylogenyen
dc.subject.meshPlant Proteinsen
dc.subject.meshPlantsen
dc.subject.meshRNA, Planten
dc.subject.meshSequence Alignmenten
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshSequence Homology, Amino Aciden
dc.titleDcAGP1, a secreted arabinogalactan protein, is related to a family of basic proline-rich proteins.en
dc.typeArticleen
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