University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Cancer Research Group > Identification of the pocket factors in a picornavirus

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/15892
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Identification of the pocket factors in a picornavirus
Authors: Smyth, M.S.
Martin, Jan H.
Pettitt, T.
Symonds, A.
Citation: Archives of Virology, 148(6): 1225-1233
Publisher: Springer Wien
Issue Date: 2003
URI: http://hdl.handle.net/2436/15892
DOI: 10.1007/s00705-002-0974-4
Additional Links: http://www.springerlink.com/content/1432-8798/?k=identification+of+the+pocket+factors
http://direct.bl.uk/bld/PlaceOrder.do?UIN=132652958&ETOC=RN&from=searchengine
Abstract: Bovine enterovirus (BEV), along with other enteroviruses and the rhinoviruses, has a hydrophobic pocket within structural protein VP1. In the crystal structures of these viruses there is electron density commensurate with a non-protein molecule within the pocket. These molecules, termed pocket factors, have been shown to stabilise the capsid and their removal from the pocket is a necessary prerequisite to uncoating. The pocket factors have been proposed, from the electron densities and uncoating studies, to be short chain fatty acids. In order to identify the pocket factor of BEV, we have grown and purified the virus in an identical manner to that used for the crystal structure determination and have performed a lipophilic extraction. Palmitic acid, C16:0, was the most abundant accounting for 40.8% by mass of the lipophilic extract (39.3 mol%). Myristic acid C14:0, was next most abundant at 18.5% by mass (20.0 mol%). In addition, we have identified other fatty acids in smaller proportions. We have therefore shown that BEV contains saturated fatty acid pocket factors of varying chain length. We have also compared the profile of the fatty acyl chain composition of BEV with those for uninfected BHK-21 cell plasma membrane and endoplasmic reticulum extracts.
Type: Article
Language: en
Description: Metadata only
Keywords: Bovine enteroviruses
Pocket factors
Picornaviruses
Biomedical science
Life science
Medicine
Animal viruses
ISSN: 03048608,14328798
Appears in Collections: Cancer Research Group

Files in This Item:

There are no files associated with this item.



All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies