University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Cancer Research Group > A novel multiplex RT-PCR system detects human endogenous retrovirus-K in breast cancer

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/15870
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: A novel multiplex RT-PCR system detects human endogenous retrovirus-K in breast cancer
Authors: Ejtehadi, H. Davari
Martin, Jan H.
Junying, J
Roden, Denise A.
Lahiri, M.
Warren, Phil
Murray, Paul G.
Nelson, Paul N.
Citation: Archives of Virology, 150(1): 177-184
Publisher: Springer Wien
Issue Date: 2005
URI: http://hdl.handle.net/2436/15870
DOI: 10.1007/s00705-004-0378-8
Additional Links: http://www.springerlink.com/content/1432-8798/?k=a+novel+multiplex+RT-PCR
Abstract: Human endogenous retrovirus HERV-K like-sequences have been implicated in certain cancers. We developed a novel multiplex RT-PCR system for HERV-K that yielded a 533bp product together with a smaller sized product (319bp) of the house keeping gene, histidyl tRNA synthetase (HtRNAS). The latter spanned an intron that also served to validate target cDNA. PCR amplicons of HERV-K and HtRNAS were visualised using a gel documentation system and the pixel intensity used to derive semi-quantitative levels of viral expression. Our data showed that HERV-K10 was significantly elevated in MCF-7 cells treated with estrogen. Interestingly, HERV-K expression was higher in MCF-7 cells selected with adriamycin. RT-PCR combined with Southern blotting also detected HERV-K from breast cancer tissue using laser capture microscopy. This study highlights the presence of HERV-K in the breast cancer cell lines MCF-7 and MCF-7 ADR and confirms HERV-K10 transcripts in the cell line T47D. We believe this study to be a novel approach in determining levels of HERV-K expression and for detecting this virus in cancer cell lines and tissues.
Type: Article
Language: en
Description: Metadata only.
Keywords: Human Endogenous Retroviruses
RT-PCR system
Biomedical science
Medicine
Life science
ISSN: 03048608,14328798
Appears in Collections: Cancer Research Group

Files in This Item:

There are no files associated with this item.



All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies