Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/2436/15833
Title:
Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.
Authors:
Yue, Patrick Y. K.; Wong, Daisy Y. L.; Ha, Wai-Yan; Fung, M.C.; Mak, Nai Ki; Yeung, H.W.; Leung, Hei Wun; Chan, Kelvin C.; Liu, Liang; Fan, T. P. David; Wong, Ricky N. S.
Abstract:
The major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.
Citation:
Angiogenesis, 8(3): 205-216
Publisher:
Springer Verlag
Issue Date:
2005
URI:
http://hdl.handle.net/2436/15833
DOI:
10.1007/s10456-005-9000-2
PubMed ID:
16328162
Additional Links:
http://www.springerlink.com/content/y77908j6248l6362/
Type:
Article
Language:
en
Description:
Metadata only
ISSN:
0969-6970
Appears in Collections:
Pharmacy and Natural Products Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorYue, Patrick Y. K.-
dc.contributor.authorWong, Daisy Y. L.-
dc.contributor.authorHa, Wai-Yan-
dc.contributor.authorFung, M.C.-
dc.contributor.authorMak, Nai Ki-
dc.contributor.authorYeung, H.W.-
dc.contributor.authorLeung, Hei Wun-
dc.contributor.authorChan, Kelvin C.-
dc.contributor.authorLiu, Liang-
dc.contributor.authorFan, T. P. David-
dc.contributor.authorWong, Ricky N. S.-
dc.date.accessioned2008-01-08T15:08:35Z-
dc.date.available2008-01-08T15:08:35Z-
dc.date.issued2005-
dc.identifier.citationAngiogenesis, 8(3): 205-216en
dc.identifier.issn0969-6970-
dc.identifier.pmid16328162-
dc.identifier.doi10.1007/s10456-005-9000-2-
dc.identifier.urihttp://hdl.handle.net/2436/15833-
dc.descriptionMetadata onlyen
dc.description.abstractThe major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.relation.urlhttp://www.springerlink.com/content/y77908j6248l6362/en
dc.subjectAngiogenesisen
dc.subjectGene Expression Profilingen
dc.subjectGinsenosidesen
dc.subjectHUVECen
dc.subjectMicroarrayen
dc.subjectRg1en
dc.titleElucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.en
dc.typeArticleen

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