Wolverhampton Intellectual Repository and E-Theses >
Research Institutes >
Research Institute in Healthcare Science >
Pharmacy and Natural Products Research Group >
Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.
this identifier to cite or link
to this item:
|Title: ||Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro.|
|Citation: ||Angiogenesis, 8(3): 205-216|
|Publisher: ||Springer Verlag|
|Issue Date: ||2005 |
|PubMed ID: ||16328162|
|Additional Links: ||http://www.springerlink.com/content/y77908j6248l6362/|
|Abstract: ||The major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.|
|Description: ||Metadata only|
Gene Expression Profiling
|Appears in Collections: ||Pharmacy and Natural Products Research Group|
|Files in This Item:|
There are no files associated with this item.
|Related articles on PubMed|
The angiosuppressive effects of 20(R)- ginsenoside Rg3.
Yue PY, Wong DY, Wu PK, Leung PY, Mak NK, Yeung HW, Liu L, Cai Z, Jiang ZH, Fan TP, Wong RN
2006 Aug 14
|See all 79 articles|
All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.