University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Cancer Research Group > Mannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress?

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/14649
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Mannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress?
Authors: Knigge, Thomas
Mann, Neelam
Parveen, Zahida
Perry, Christopher
Gernhöfer, Maike
Triebskorn, Rita
Köhler, Heinz-R
Connock, Martin
Citation: Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology 2002, 131(3): 259-269
Publisher: Elsevier BV
Issue Date: 2002
URI: http://hdl.handle.net/2436/14649
DOI: 10.1016/S1532-0456(02)00013-3
Additional Links: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W89-452F7N2-3&_user=1644469&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000054077&_version=1&_urlVersion=0&_userid=1644469&md5=562af2dc8f70808ea43c318ede896f8b
Abstract: Amongst animals, several hydrogen peroxide-generating oxidases are apparently restricted to molluscs. One of these, -mannitol oxidase, is concentrated in the alimentary system, where it is associated with its own subcellular membrane system of unique tubular morphology, most likely representing a structural modification of the ER. These structures can be purified by subcellular fractionation and have been termed ‘mannosomes’. Little is known about the functions of mannitol oxidase or of mannosomes, but the previously reported molluscicide-induced increase in mannosomes implies their involvement in a general stress reaction. In this study, we examined the effects of heavy metal stress in the terrestrial gastropod Arion lusitanicus. The activity of mannitol oxidase and mannosome abundance were monitored, together with metal effects on heat-shock protein level, and these parameters were compared to heavy metal accumulation in the digestive gland. We found that mannitol oxidase is inhibited by heavy metals more than other oxidases. On the other hand, hsp70 levels and mannosomal protein were increased with enhanced heavy metal stress, the latter indicating a probable increase in the number of mannosome organelles. Thus, stress protein (hsp70) and mannosomal protein were positively correlated with heavy metal accumulation, whereas the enzyme activity showed a negative correlation with increasing heavy metal content of the slugs.
Type: Article
Language: en
Description: Metadadata only
Keywords: Arion lusitanicus
Slug
Digestive gland
Generating oxidases
Enzyme
Gastropoda
Heat-shock protein (hsp70)
Epatopancreas
Organelle
H2O2-generating oxidases
ISSN: 15320456
Appears in Collections: Cancer Research Group

Files in This Item:

There are no files associated with this item.



All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies