2.50
Hdl Handle:
http://hdl.handle.net/2436/14646
Title:
Novel mastoparan analogs induce differential secretion from mast cells.
Authors:
Farquhar, Michelle; Soomets, Ursel; Bates, Ruth L.; Martin, Ashley; Langel, Ulo; Howl, John D.
Abstract:
Cationic amphiphilic peptides stimulate secretion via a receptor-independent action upon G proteins. We have previously utilized chimeric analogs of mastoparan (MP), including galparan (galanin(1-13)-MP ), as molecular probes of secretion. Here, we further resolve the structure-activity relationship of peptidyl secretagogs, including rationally designed chimeric MP analogs. The secretory efficacies of 10 MP analogs were significantly higher than 45 unrelated basic peptides. Comparative studies identified MP analogs that are differential secretagogs for 5-hydroxytryptamine (5-HT) and beta-hexosaminidase. Peptide-induced activation of phospholipase D (PLD), an enzyme intimately involved in regulated exocytosis [5], correlated with the secretion of beta-hexosaminidase but not 5-HT. Thus, these data indicate that different mechanisms are responsible for the exocytosis of 5-HT and beta-hexosaminidase, respectively. Moreover, mastoparan analogs are novel tools for probing the molecular details of exocytosis and other biological phenomena.
Citation:
Chemistry & Biology, 9(1): 63-70
Publisher:
Elsevier Science B.V
Issue Date:
2002
URI:
http://hdl.handle.net/2436/14646
DOI:
10.1016/S1074-5521(01)00098-9
Additional Links:
http://www.ingentaconnect.com/content/els/10745521/2002/00000009/00000001/art00098; http://direct.bl.uk/bld/PlaceOrder.do?UIN=108952570&ETOC=RN&from=searchengine
Type:
Article
Language:
en
Description:
Metadata only
ISSN:
10745521
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorFarquhar, Michelle-
dc.contributor.authorSoomets, Ursel-
dc.contributor.authorBates, Ruth L.-
dc.contributor.authorMartin, Ashley-
dc.contributor.authorLangel, Ulo-
dc.contributor.authorHowl, John D.-
dc.date.accessioned2007-11-19T12:19:32Z-
dc.date.available2007-11-19T12:19:32Z-
dc.date.issued2002-
dc.identifier.citationChemistry & Biology, 9(1): 63-70en
dc.identifier.issn10745521-
dc.identifier.doi10.1016/S1074-5521(01)00098-9-
dc.identifier.urihttp://hdl.handle.net/2436/14646-
dc.descriptionMetadata onlyen
dc.description.abstractCationic amphiphilic peptides stimulate secretion via a receptor-independent action upon G proteins. We have previously utilized chimeric analogs of mastoparan (MP), including galparan (galanin(1-13)-MP ), as molecular probes of secretion. Here, we further resolve the structure-activity relationship of peptidyl secretagogs, including rationally designed chimeric MP analogs. The secretory efficacies of 10 MP analogs were significantly higher than 45 unrelated basic peptides. Comparative studies identified MP analogs that are differential secretagogs for 5-hydroxytryptamine (5-HT) and beta-hexosaminidase. Peptide-induced activation of phospholipase D (PLD), an enzyme intimately involved in regulated exocytosis [5], correlated with the secretion of beta-hexosaminidase but not 5-HT. Thus, these data indicate that different mechanisms are responsible for the exocytosis of 5-HT and beta-hexosaminidase, respectively. Moreover, mastoparan analogs are novel tools for probing the molecular details of exocytosis and other biological phenomena.en
dc.language.isoenen
dc.publisherElsevier Science B.Ven
dc.relation.urlhttp://www.ingentaconnect.com/content/els/10745521/2002/00000009/00000001/art00098en
dc.relation.urlhttp://direct.bl.uk/bld/PlaceOrder.do?UIN=108952570&ETOC=RN&from=searchengine-
dc.subjectNovel mastoparan analogsen
dc.subjectDifferential secretionen
dc.subjectMast Cellsen
dc.subjectPeptides-
dc.titleNovel mastoparan analogs induce differential secretion from mast cells.en
dc.typeArticleen
dc.format.digYES-
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