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Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Diabetes, Physiology and Molecular Medicine Research Group > Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/113828
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Title: Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells.
Authors: Brown, James E. P.
Onyango, David J.
Ramanjaneya, Manjunath
Conner, Alex C.
Patel, Snehal T.
Dunmore, Simon J.
Randeva, Harpal S.
Citation: Journal of molecular endocrinology, 44 (3):171-178
Publisher: Society for Endocinology
Journal: Journal of molecular endocrinology
Issue Date: 2010
URI: http://hdl.handle.net/2436/113828
DOI: 10.1677/JME-09-0071
PubMed ID: 19906834
Abstract: The role of the adipocyte-derived factor visfatin in metabolism remains controversial, although some pancreatic beta-cell-specific effects have been reported. This study investigated the effects of visfatin upon insulin secretion, insulin receptor activation and mRNA expression of key diabetes-related genes in clonal mouse pancreatic beta-cells. beta-TC6 cells were cultured in RPMI 1640 and were subsequently treated with recombinant visfatin. One-hour static insulin secretion was measured by ELISA. Phospho-specific ELISA and western blotting were used to detect insulin receptor activation. Real-time SYBR Green PCR array technology was used to measure the expression of 84 diabetes-related genes in both treatment and control cells. Incubation with visfatin caused significant changes in the mRNA expression of several key diabetes-related genes, including marked up-regulation of insulin (9-fold increase), hepatocyte nuclear factor (HNF)1beta (32-fold increase), HNF4alpha (16-fold increase) and nuclear factor kappaB (40-fold increase). Significant down-regulation was seen in angiotensin-converting enzyme (-3.73-fold) and UCP2 (-1.3-fold). Visfatin also caused a significant 46% increase in insulin secretion compared to control (P<0.003) at low glucose, and this increase was blocked by co-incubation with the specific nicotinamide phosphoribosyltransferase inhibitor FK866. Both visfatin and nicotinamide mononucleotide induced activation of both insulin receptor and extracellular signal-regulated kinase (ERK)1/2, with visfatin-induced insulin receptor/ERK1/2 activation being inhibited by FK866. We conclude that visfatin can significantly regulate insulin secretion, insulin receptor phosphorylation and intracellular signalling and the expression of a number of beta-cell function-associated genes in mouse beta-cells.
Type: Article
Language: en
MeSH: Animals
Cell Line
Diabetes Mellitus
Insulin
Insulin-Secreting Cells
Mice
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Nicotinamide Phosphoribosyltransferase
Polymerase Chain Reaction
RNA, Messenger
Receptor, Insulin
Signal Transduction
ISSN: 1479-6813
Appears in Collections: Diabetes, Physiology and Molecular Medicine Research Group

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