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Title: Pseudoislets as primary islet replacements for research: Report on a symposium at King's College London, London UK
Authors: Persaud, Shanta
Arden, Catherine
Bergsten, P.
Bone, Adrian J.
Brown, James
Dunmore, Simon J
Harrison, Moira
Hauge-Evans, Astrid
Kelly, Catriona
King, Aileen
Maffucci, Tania
Marriott, Claire E.
McClenaghan, Neville
Morgan, Noel G.
Reers, Christina
Russell, Mark A.
Turner, Mark D.
Willoughby, Emma
Younis, MustafaY.G.
Zhi, Z.L.
Jones, P.M.
Citation: Islets, 2(4):236-239
Publisher: Landes Bioscience
Journal: Islets
Issue Date: 2010
DOI: 10.4161/isl.2.4.12557
Additional Links:
Abstract: Laboratory-based research aimed at understanding processes regulating insulin secretion and mechanisms underlying β-cell dysfunction and loss in diabetes often makes use of rodents, as these processes are in many respects similar between rats/mice and humans. Indeed, a rough calculation suggests that islets have been isolated from as many as 150,000 rodents to generate the data contained within papers published in 2009 and the first four months of 2010. Rodent use for islet isolation has been mitigated, to a certain extent, by the availability of a variety of insulin-secreting cell lines that are used by researchers world-wide. However, when maintained as monolayers the cell lines do not replicate the robust, sustained secretory responses of primary islets which limits their usefulness as islet surrogates. On the other hand, there have been several reports that configuration of MIN6 β-cells, derived from a mouse insulinoma, as three-dimensional cell clusters termed ‘pseudoislets’ largely recapitulates the function of primary islet β-cells. The Diabetes Research Group at King’s College London has been using the MIN6 pseudoislet model for over a decade and they hosted a symposium on “Pseudoislets as primary islet replacements for research”, which was funded by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), in London on 15th and 16th April 2010. This small, focused meeting was conceived as an opportunity to consolidate information on experiences of working with pseudoislets between different UK labs, and to introduce the theory and practice of pseudoislet culture to laboratories working with islets and/or β-cell lines but who do not currently use pseudoislets. This short review summarizes the background to the development of the cell line-derived pseudoislet model, the key messages arising from the symposium and emerging themes for future pseudoislet research
Type: Article
Language: en
Keywords: β-cell function
Islet substitutes
MIN6 cells
Insulin secretion
ISSN: 1938-2014
Appears in Collections: Diabetes, Physiology and Molecular Medicine Research Group

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