University of Wolverhampton
Browse
Collection All
bullet
bullet
bullet
bullet
Listed communities
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet
bullet

Wolverhampton Intellectual Repository and E-Theses > Research Institutes > Research Institute in Healthcare Science > Molecular Pharmacology Research Group > Inorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.

Please use this identifier to cite or link to this item: http://hdl.handle.net/2436/11143
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!



Title: Inorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.
Authors: Zhang, Haiyu
Gómez-García, Maria R.
Brown, Michael R. W.
Kornberg, Arthur
Citation: Proc. Natl. Acad. Sci. U.S.A. 2005, 102(8):2731-5
Publisher: National Academy of Sciences
Issue Date: 2005
URI: http://hdl.handle.net/2436/11143
DOI: 10.1073/pnas.0500023102
PubMed ID: 15701689
Additional Links: http://www.pnas.org/content/102/8/2731
Abstract: Dictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and for nutritional predation on bacteria. The synthesis of poly P, a polymer of tens or hundreds of phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria by poly P kinase (PPK1). The eukaryote D. discoideum possesses a homolog of PPK1. We report here that mutants of D. discoideum PPK1 (DdPPK1) have reduced levels of poly P and are deficient in development. Fruiting bodies are smaller and produce fewer spores, which appear to germinate like the wild type (WT). The DdPPK1 mutant formed smaller plaques on bacterial lawns compared with those of the WT. Predation by D. discoideum, assessed by uptake and digestion of Klebsiella aerogenes, showed that fewer bacteria were taken up by the DdPPK1 mutant compared with the WT and were killed less rapidly, indicating a role of poly P and/or DdPPK1 in phagocytosis. On Pseudomonas aeruginosa lawns, cleared plaques were observed with the bacterial PPK1 mutant but not with the WT P. aeruginosa. Thus, poly P is important in predation both for the predator and prey.
Type: Article
Language: en
Keywords: Dictyostelium discoideum
Inorganic polyphosphate
Sporulation
Predation
ISSN: 0027-8424
Appears in Collections: Molecular Pharmacology Research Group

Files in This Item:
File Description Size Format View/Open
Dicty AK 05 2731.pdf343KbAdobe PDFThumbnail
View/Open

Related articles on PubMed
bullet
bullet
bullet
bullet
bullet
See all 174 articles

All Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Fairtrade - Guarantees a better deal for Third World Producers

University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY

Course enquiries: 0800 953 3222, General enquiries: 01902 321000,
Email: enquiries@wlv.ac.uk | Freedom of Information | Disclaimer and copyright | Website feedback | The University as a charity

OR Logo Powered by Open Repository | Cookies