Inorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.

2.50
Hdl Handle:
http://hdl.handle.net/2436/11143
Title:
Inorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.
Authors:
Zhang, Haiyu; Gómez-García, Maria R.; Brown, Michael R. W.; Kornberg, Arthur
Abstract:
Dictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and for nutritional predation on bacteria. The synthesis of poly P, a polymer of tens or hundreds of phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria by poly P kinase (PPK1). The eukaryote D. discoideum possesses a homolog of PPK1. We report here that mutants of D. discoideum PPK1 (DdPPK1) have reduced levels of poly P and are deficient in development. Fruiting bodies are smaller and produce fewer spores, which appear to germinate like the wild type (WT). The DdPPK1 mutant formed smaller plaques on bacterial lawns compared with those of the WT. Predation by D. discoideum, assessed by uptake and digestion of Klebsiella aerogenes, showed that fewer bacteria were taken up by the DdPPK1 mutant compared with the WT and were killed less rapidly, indicating a role of poly P and/or DdPPK1 in phagocytosis. On Pseudomonas aeruginosa lawns, cleared plaques were observed with the bacterial PPK1 mutant but not with the WT P. aeruginosa. Thus, poly P is important in predation both for the predator and prey.
Citation:
Proc. Natl. Acad. Sci. U.S.A. 2005, 102(8):2731-5
Publisher:
National Academy of Sciences
Issue Date:
2005
URI:
http://hdl.handle.net/2436/11143
DOI:
10.1073/pnas.0500023102
PubMed ID:
15701689
Additional Links:
http://www.pnas.org/content/102/8/2731
Submitted date:
2007-01-26
Type:
Article
Language:
en
ISSN:
0027-8424
Appears in Collections:
Molecular Pharmacology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Haiyu-
dc.contributor.authorGómez-García, Maria R.-
dc.contributor.authorBrown, Michael R. W.-
dc.contributor.authorKornberg, Arthur-
dc.date.accessioned2007-04-05T11:34:44Z-
dc.date.available2007-04-05T11:34:44Z-
dc.date.issued2005-
dc.date.submitted2007-01-26-
dc.identifier.citationProc. Natl. Acad. Sci. U.S.A. 2005, 102(8):2731-5en
dc.identifier.issn0027-8424-
dc.identifier.pmid15701689-
dc.identifier.doi10.1073/pnas.0500023102-
dc.identifier.urihttp://hdl.handle.net/2436/11143-
dc.description.abstractDictyostelium discoideum, a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and for nutritional predation on bacteria. The synthesis of poly P, a polymer of tens or hundreds of phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria by poly P kinase (PPK1). The eukaryote D. discoideum possesses a homolog of PPK1. We report here that mutants of D. discoideum PPK1 (DdPPK1) have reduced levels of poly P and are deficient in development. Fruiting bodies are smaller and produce fewer spores, which appear to germinate like the wild type (WT). The DdPPK1 mutant formed smaller plaques on bacterial lawns compared with those of the WT. Predation by D. discoideum, assessed by uptake and digestion of Klebsiella aerogenes, showed that fewer bacteria were taken up by the DdPPK1 mutant compared with the WT and were killed less rapidly, indicating a role of poly P and/or DdPPK1 in phagocytosis. On Pseudomonas aeruginosa lawns, cleared plaques were observed with the bacterial PPK1 mutant but not with the WT P. aeruginosa. Thus, poly P is important in predation both for the predator and prey.en
dc.format.extent351694 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.publisherNational Academy of Sciencesen
dc.relation.urlhttp://www.pnas.org/content/102/8/2731en
dc.subjectDictyostelium discoideumen
dc.subjectInorganic polyphosphateen
dc.subjectSporulationen
dc.subjectPredationen
dc.titleInorganic polyphosphate in Dictyostelium discoideum: influence on development, sporulation, and predation.en
dc.typeArticleen
dc.format.digYES-

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