http://wlv.openrepository.com:80/wlv WIRE captures, stores, indexes, preserves, and distributes digital research material. Sat, 25 May 2013 18:49:24 GMT 2013-05-25T18:49:24Z http://hdl.handle.net/2436/279612 Title: Detergent effects on disinfectant susceptibility of Escherichia coli and listeria monocytogenes attached to stainless steel Authors: Walton, Julie Abstract: This study investigated the effect of detergent treatment on susceptibility of attached Escherichia coli and Listeria monocytogenes to subsequent disinfectant treatment, in relation to food industry cleaning procedures. E. coli attached to stainless steel surfaces became significantly more susceptible to benzalkonium chloride (BAC) after treatment with sodium alkyl sulphate (SAS) by 0.51 Log10 cfu ml-1 and fatty alcohol ethoxylate (FAE) by 0.96 Log10 cfu ml-1. No change in susceptibility was observed with sodium dodecyl sulphate (SDS), sodium lauryl ethyl sulphate (SLES) or polyethoxylated alcohol (PEA). L. monocytogenes became significantly less susceptible to BAC after treatment with anionic detergents SAS by 0.79 Log10 cfu ml-1, SDS by 0.33 Log10 cfu ml-1 and SLES by 0.22 Log10 cfu ml-1, yet no change in susceptibility was observed with FAE. Following treatment with all detergents both organisms became significantly more susceptible to sodium dichloroisocyanurate (NaDCC) demonstrating that the effect of the disinfectant was independent of detergent type. Flow cytometry using the fluorochrome propidium iodide (PI) revealed significant increases in cell membrane permeability of both organisms by all detergents except sodium dodecyl sulphate (SDS) and the effect was much greater in E. coli. Increasing above the in-use concentration of SAS and FAE had no further effect on cell membrane permeability, or susceptibility to BAC. Hydrophobic interaction chromatography (HIC) showed that E. coli became less hydrophobic following treatment with SAS, SDS, FAE and L. monocytogenes became less hydrophobic following treatment with SAS and SDS but no effect was seen with FAE. Investigations into carbon chain length of detergent revealed that SAS and the C18 standard increased susceptibility of E. coli to BAC which, with permeability results, suggests a link between increase in susceptibility to BAC and increase in membrane permeability. Efflux experiments with L. monocytogenes showed that efflux of ethidium bromide (EtBr) was greater from cells treated with SAS than with FAE suggesting that the anionic charge on the detergent molecule influences an efflux mechanism that reduces susceptibility to BAC. Overall the results demonstrate that detergent type can influence the sensitivity of persistent food borne microorganisms to BAC and NaDCC and the significance of the findings may impact on the choice of agents used in cleaning procedures in the food industry. Description: A thesis submitted in partial fulfilment of the requirements of the University of Wolverhampton for the degree of Doctor of Philosophy Sun, 01 Jul 2012 00:00:00 GMT http://hdl.handle.net/2436/279612 2012-07-01T00:00:00Z http://hdl.handle.net/2436/268772 Title: Researching an overlooked workforce in a University: catering, caretaking and securtiy Authors: Meakin, Susan Elizabeth Abstract: A thesis submitted in partial fulfilment of the requirements University of Wolverhampton Degree of Doctor of Philosophy Sun, 01 Jan 2012 00:00:00 GMT http://hdl.handle.net/2436/268772 2012-01-01T00:00:00Z http://hdl.handle.net/2436/268058 Title: Towards auto/pedagogy:A reflexive auto/biographic case study of professional learning mediated by technology Authors: Hughes, Simon Abstract: Tracing the development of my thinking and professional practice from the late 1980s to the present day, this thesis uses the auto/biographic method developed at CCCU by Linden West (e.g. West, 2004), in combination with a personal, reflexive dialogic hermeneutic redolent of the epistemological approaches of liberation theologians in the 1970s and 1980s, to evaluate critically the influence of five illustrative moments on the generation of new knowledge. The thesis argues that demonstrable learning gains were made when particular factors came together in these autobiographical moments. The insertion of the slash "/" in auto/biography denotes the self-directed reflection on these narratives, carried out systematically in order to derive meaning from them. The process of critical reflection on the narratives interwoven with reading around epistemology, the self and (information) technology, led to a framework emerging. Within the illustrative moments there appear to be four factors that, in combination, cause learning to occur: need, knowledge, networks and the application of newly-acquired knowledge in a new context or setting. Phonically, the framework can be argued to be N4. I argue that in the 21st Century, especially where what is to be learned is something technological, learning most likely occurs when all these factors are present. Recognising this to be a personal phenomenon I adopt the term auto/pedagogy to describe it since I believe that learning is a personal commitment to changing the state of one's being. Wed, 10 Oct 2012 00:00:00 GMT http://hdl.handle.net/2436/268058 2012-10-10T00:00:00Z http://hdl.handle.net/2436/267852 Title: Neonatal Nightingales: Live Parental and Neonatal Nurse Infant-Directed Singing as a Beneficial Intervention for the Health and Development of Infants in Neonatal Care Authors: Barnell, Ruby Abstract: It is the aim of this study to establish live neonatal nurse and parental infant-directed singing as a potentially efficacious intervention that is beneficial to the health and development of infants in neonatal care and which could conceivably be integrated into nursing practice and the neonatal care environment in a way that is both practical and viable. An appraisal of extant literature highlights the need for further intervention in neonatal care, explains how music meets the necessary criterion as appropriate intervention and discusses its potential, specifically infant-directed singing, to be provided by neonatal nurses and parents. The willingness of neonatal nurses and parents to engage in infant-directed singing in the neonatal care unit environment is assessed through an informal questionnaire survey. From the findings of this and other studies, implications and recommendations for both areas and methods for future research are extrapolated. The intention behind this study is to generate interest and awareness of academics and medical professionals in this potentially cost effective intervention, promoting future research and practice. It is hoped that this will ultimately result in the comfort of song providing profound benefits to the physiological, psychological and emotional development and wellbeing of all infants in neonatal care. Description: A Project submitted in partial fulfilment of the assessment requirements for the award of MA Musicology Sun, 01 Jan 2012 00:00:00 GMT http://hdl.handle.net/2436/267852 2012-01-01T00:00:00Z